TY - JOUR
T1 - Biodegradable chitosan-graft-poly(l-lactide) copolymers for bone tissue engineering
AU - Kaliva, Maria
AU - Georgopoulou, Anthie
AU - Dragatogiannis, Dimitrios A.
AU - Charitidis, Costas A.
AU - Chatzinikolaidou, Maria
AU - Vamvakaki, Maria
N1 - Funding Information:
Funding: This research was funded by the General Secretariat for Research and Technology (GSRT) grant “Aristeia II” MIS 525089 entitled: “In vitro assessment of osteoinductive biomimetic and polymeric composite biomaterial scaffolds for bone tissue repair; OSTEOBIOMIMESIS 3438.” Acknowledgments: The authors would like to acknowledge Aleka Manousaki for technical assistance with the SEM and AFM measurements.
Publisher Copyright:
© 2020 by the authors.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - The design and synthesis ofnewbiomaterials with adjustable physicochemical and biological properties for tissue engineering applications have attracted great interest. In this work, chitosan-graftpoly(l-lactide) (CS-g-PLLA) copolymers were prepared by chemically binding poly(L-lactide) (PLLA) chains along chitosan (CS) via the "grafting to" approach to obtain hybrid biomaterials that present enhanced mechanical stability, due to the presence of PLLA, and high bioactivity, conferred by CS. Two graft copolymers were prepared, CS-g-PLLA(80/20) and CS-g-PLLA(50/50), containing 82 wt % and 55 wt % CS, respectively. Degradation studies of compressed discs of the copolymers showed that the degradation rate increased with the CS content of the copolymer. Nanomechanical studies in the dry state indicated that the copolymer with the higher CS content had larger Young modulus, reduced modulus and hardness values, whereas the moduli and hardness decreased rapidly following immersion of the copolymer discs in alpha-MEM cell culture medium for 24 h. Finally, the bioactivity of the hybrid copolymers was evaluated in the adhesion and growth of MC3T3-E1 pre-osteoblastic cells. In vitro studies showed that MC3T3-E1 cells exhibited strong adhesion on both CS-g-PLLA graft copolymer films from the first day in cell culture, whereas the copolymer with the higher PLLA content, CS-g-PLLA(50/50), supported higher cell growth.
AB - The design and synthesis ofnewbiomaterials with adjustable physicochemical and biological properties for tissue engineering applications have attracted great interest. In this work, chitosan-graftpoly(l-lactide) (CS-g-PLLA) copolymers were prepared by chemically binding poly(L-lactide) (PLLA) chains along chitosan (CS) via the "grafting to" approach to obtain hybrid biomaterials that present enhanced mechanical stability, due to the presence of PLLA, and high bioactivity, conferred by CS. Two graft copolymers were prepared, CS-g-PLLA(80/20) and CS-g-PLLA(50/50), containing 82 wt % and 55 wt % CS, respectively. Degradation studies of compressed discs of the copolymers showed that the degradation rate increased with the CS content of the copolymer. Nanomechanical studies in the dry state indicated that the copolymer with the higher CS content had larger Young modulus, reduced modulus and hardness values, whereas the moduli and hardness decreased rapidly following immersion of the copolymer discs in alpha-MEM cell culture medium for 24 h. Finally, the bioactivity of the hybrid copolymers was evaluated in the adhesion and growth of MC3T3-E1 pre-osteoblastic cells. In vitro studies showed that MC3T3-E1 cells exhibited strong adhesion on both CS-g-PLLA graft copolymer films from the first day in cell culture, whereas the copolymer with the higher PLLA content, CS-g-PLLA(50/50), supported higher cell growth.
KW - Bone tissue engineering
KW - Chitosan
KW - CS-g-PLLA
KW - Graft copolymers
KW - MC3T3-E1
KW - Poly(l-lactide)
KW - Preosteoblastic cells
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U2 - 10.3390/polym12020316
DO - 10.3390/polym12020316
M3 - Article
AN - SCOPUS:85081203622
VL - 12
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 2
M1 - 316
ER -