Biocompatible materials based on self-assembling peptides on Ti25Nb10Zr alloy

Molecular structure and organization investigated by synchrotron radiation induced techniques

Valeria Secchi, Stefano Franchi, Marta Santi, Alina Vladescu, Mariana Braic, Tomáš Skála, Jaroslava Nováková, Monica Dettin, Annj Zamuner, Giovanna Iucci, Chiara Battocchio

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this work, we applied advanced Synchrotron Radiation (SR) induced techniques to the study of the chemisorption of the Self Assembling Peptide EAbuK16, i.e., H-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-NH2 that is able to spontaneously aggregate in anti-parallel β-sheet conformation, onto annealed Ti25Nb10Zr alloy surfaces. This synthetic amphiphilic oligopeptide is a good candidate to mimic extracellular matrix for bone prosthesis, since its β-sheets stack onto each other in a multilayer oriented nanostructure with internal pores of 5–200 nm size. To prepare the biomimetic material, Ti25Nb10Zr discs were treated with aqueous solutions of EAbuK16 at different pH values. Here we present the results achieved by performing SR-induced X-ray Photoelectron Spectroscopy (SR-XPS), angle-dependent Near Edge X-ray Absorption Fine Structure (NEXAFS) spectroscopy, FESEM and AFM imaging on Ti25Nb10Zr discs after incubation with self-assembling peptide solution at five different pH values, selected deliberately to investigate the best conditions for peptide immobilization.

Original languageEnglish
Article number148
JournalNanomaterials
Volume8
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018
Externally publishedYes

Fingerprint

Biocompatible Materials
Synchrotron radiation
Biomaterials
Molecular structure
Peptides
Biomimetic materials
X ray absorption near edge structure spectroscopy
Chemisorption
Oligopeptides
Conformations
Nanostructures
Bone
Multilayers
X ray photoelectron spectroscopy
Imaging techniques
gamma-glutamyl-alpha-aminobutyrate

Keywords

  • Bioactive materials
  • Nanostructures
  • NEXAFS
  • Self-assembling peptides
  • Synchrotron radiation induced spectroscopies
  • Titanium alloy
  • XPS

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

Cite this

Biocompatible materials based on self-assembling peptides on Ti25Nb10Zr alloy : Molecular structure and organization investigated by synchrotron radiation induced techniques. / Secchi, Valeria; Franchi, Stefano; Santi, Marta; Vladescu, Alina; Braic, Mariana; Skála, Tomáš; Nováková, Jaroslava; Dettin, Monica; Zamuner, Annj; Iucci, Giovanna; Battocchio, Chiara.

In: Nanomaterials, Vol. 8, No. 3, 148, 01.03.2018.

Research output: Contribution to journalArticle

Secchi, Valeria ; Franchi, Stefano ; Santi, Marta ; Vladescu, Alina ; Braic, Mariana ; Skála, Tomáš ; Nováková, Jaroslava ; Dettin, Monica ; Zamuner, Annj ; Iucci, Giovanna ; Battocchio, Chiara. / Biocompatible materials based on self-assembling peptides on Ti25Nb10Zr alloy : Molecular structure and organization investigated by synchrotron radiation induced techniques. In: Nanomaterials. 2018 ; Vol. 8, No. 3.
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