Biologisierung von implantaten mit biomolekülen: Oberflächenveredelung von metallen durch behandlung mit chromschwefelsäure

Translated title of the contribution: Biocoating of implants with mediator molecules: Surface enhancement of metals by treatment with chromosulfuric acid

H. P. Jennissen, T. Zumbrink, M. Chatzinikolaidou, J. Steppuhn

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Proteins such as the model protein 125I-ubiquitin and recombinant human bone morphogenetic protein 2 (125I-rhBMP-2) were covalently immobilized on titanium surfaces after reaction of the latter with aminopropyltriethoxysilane (APS) and activation with carbonyldiimidazole (CDI). On titanium powder (grain size ∼ 60 μm, 2400 cm2/g) net amounts of 15-60 ng/cm2 of 125I-ubiquitin could be coupled after correction for ca. 25% non-specifically adsorbed protein. Similarly, normal polished and special surface-enhanced titanium and stainless steel plates (size 1 × 10 × 15 mm) were modified as above and coupled with 125I-ubiquitin and 125I-BMP-2. The surface-enhanced metal plates were prepared by a special chromosulfuric acid treatment, probably leading to an etching of the metal surface and to an increase in the thickness of the metal oxide layer, which produced a strong enhancement of the binding capacity for chemical modification reactions. Dynamic contact angle measurements (Wilhelmy) of the clean surface-enhanced plates yielded an advancing contact angle of 18.0 and zero contact angle hysteresis. On such surface-enhanced titanium plates net amounts of 300-500 ng/cm2 of 125I-ubiquitin could be immobilized, values 2-5 fold higher than obtained on normal polished plates. In comparison 60-100ng/ cm2 125I-rhBMP-2 could be immobilized on the oxidized plates. The above technique is being applied as a tool for biocoating bone implants with the aim of constructing bone-cell-reactive interfaces with specific biorecognition properties.

Translated title of the contributionBiocoating of implants with mediator molecules: Surface enhancement of metals by treatment with chromosulfuric acid
Original languageGerman
Pages (from-to)838-845
Number of pages8
JournalMaterialwissenschaft und Werkstofftechnik
Volume30
Issue number12
DOIs
Publication statusPublished - Dec 1999
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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