Atypical PKCζ transduces electrophilic fatty acid signaling in pulmonary epithelial cells

Chang Jiang Guo, Francisco J. Schopfer, Linda Gonzales, Ping Wang, Bruce A. Freeman, Andrew J. Gow

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nitric oxide and secondary oxides of nitrogen react with unsaturated fatty acids such as linoleic acid to yield oxidized and nitrated products. Fatty acid nitroalkene derivatives, (e.g. nitrolinoleate [LNO 2]) are produced by oxidative inflammatory reactions, detected clinically, display potent electrophilic reactivity and induce post-translational protein modifications that mediate adaptive inflammatory signaling responses. LNO 2 signaling was examined in lung epithelial cells because the alveolar compartment is a rich site for the transduction of redox and inflammatory reactions. LNO 2 did not directly induce Ca 2+ influx in cultured lung epithelial cells, but inhibited bradykinin-induced Ca 2+ influx in a cGMP-independent manner. In contrast, LNO 2 activated MAP kinase (Erk1/2) by a mechanism independent of bradykinin. It was hypothesized that these unique responses were transduced by activation of different protein kinase C isotypes, supported by the observation that LNO 2-mediated inhibition of Ca 2+ influx was blocked by the non-selective PKC inhibitors chelerythine chloride and calphostin C, but not by the calcium dependent "classic" PKC inhibitor Gö6976. Western blot analysis showed that atypical PKCζ was activated by LNO 2 stimulation, with PKCζ and Erk activation also demonstrated in primary culture of human lung type II cells. Addition of pseudotypical PKCζ substrate peptide reversed LNO 2-mediated induction of Ca 2+ influx and MAP kinase activation. Finally, the electrophilic nature of LNO 2 resulted in a novel mode of PKCζ activation, covalent adduction of the enzyme. In summary, LNO 2 mediated signaling in lung type II epithelial cells occurs via a unique pathway involving PKCζ.

Original languageEnglish
Pages (from-to)366-372
Number of pages7
JournalNitric Oxide - Biology and Chemistry
Volume25
Issue number3
DOIs
Publication statusPublished - 30 Oct 2011
Externally publishedYes

Fingerprint

Fatty Acids
Epithelial Cells
Chemical activation
Lung
Mitogen-Activated Protein Kinase 1
Bradykinin
Nitrogen Oxides
Alveolar Epithelial Cells
Linoleic Acid
Post Translational Protein Processing
Unsaturated Fatty Acids
Protein Kinase C
Oxidation-Reduction
Chlorides
Nitric Oxide
Phosphotransferases
Western Blotting
Calcium
Derivatives
Peptides

Keywords

  • Calcium mobilization
  • Nitrated lipids
  • Protein kinase C
  • Pulmonary epithelial cell
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cancer Research
  • Physiology

Cite this

Atypical PKCζ transduces electrophilic fatty acid signaling in pulmonary epithelial cells. / Guo, Chang Jiang; Schopfer, Francisco J.; Gonzales, Linda; Wang, Ping; Freeman, Bruce A.; Gow, Andrew J.

In: Nitric Oxide - Biology and Chemistry, Vol. 25, No. 3, 30.10.2011, p. 366-372.

Research output: Contribution to journalArticle

Guo, Chang Jiang ; Schopfer, Francisco J. ; Gonzales, Linda ; Wang, Ping ; Freeman, Bruce A. ; Gow, Andrew J. / Atypical PKCζ transduces electrophilic fatty acid signaling in pulmonary epithelial cells. In: Nitric Oxide - Biology and Chemistry. 2011 ; Vol. 25, No. 3. pp. 366-372.
@article{a92dbf581a3642f9997363979589a895,
title = "Atypical PKCζ transduces electrophilic fatty acid signaling in pulmonary epithelial cells",
abstract = "Nitric oxide and secondary oxides of nitrogen react with unsaturated fatty acids such as linoleic acid to yield oxidized and nitrated products. Fatty acid nitroalkene derivatives, (e.g. nitrolinoleate [LNO 2]) are produced by oxidative inflammatory reactions, detected clinically, display potent electrophilic reactivity and induce post-translational protein modifications that mediate adaptive inflammatory signaling responses. LNO 2 signaling was examined in lung epithelial cells because the alveolar compartment is a rich site for the transduction of redox and inflammatory reactions. LNO 2 did not directly induce Ca 2+ influx in cultured lung epithelial cells, but inhibited bradykinin-induced Ca 2+ influx in a cGMP-independent manner. In contrast, LNO 2 activated MAP kinase (Erk1/2) by a mechanism independent of bradykinin. It was hypothesized that these unique responses were transduced by activation of different protein kinase C isotypes, supported by the observation that LNO 2-mediated inhibition of Ca 2+ influx was blocked by the non-selective PKC inhibitors chelerythine chloride and calphostin C, but not by the calcium dependent {"}classic{"} PKC inhibitor G{\"o}6976. Western blot analysis showed that atypical PKCζ was activated by LNO 2 stimulation, with PKCζ and Erk activation also demonstrated in primary culture of human lung type II cells. Addition of pseudotypical PKCζ substrate peptide reversed LNO 2-mediated induction of Ca 2+ influx and MAP kinase activation. Finally, the electrophilic nature of LNO 2 resulted in a novel mode of PKCζ activation, covalent adduction of the enzyme. In summary, LNO 2 mediated signaling in lung type II epithelial cells occurs via a unique pathway involving PKCζ.",
keywords = "Calcium mobilization, Nitrated lipids, Protein kinase C, Pulmonary epithelial cell, Signal transduction",
author = "Guo, {Chang Jiang} and Schopfer, {Francisco J.} and Linda Gonzales and Ping Wang and Freeman, {Bruce A.} and Gow, {Andrew J.}",
year = "2011",
month = "10",
day = "30",
doi = "10.1016/j.niox.2011.07.003",
language = "English",
volume = "25",
pages = "366--372",
journal = "Nitric Oxide - Biology and Chemistry",
issn = "1089-8603",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Atypical PKCζ transduces electrophilic fatty acid signaling in pulmonary epithelial cells

AU - Guo, Chang Jiang

AU - Schopfer, Francisco J.

AU - Gonzales, Linda

AU - Wang, Ping

AU - Freeman, Bruce A.

AU - Gow, Andrew J.

PY - 2011/10/30

Y1 - 2011/10/30

N2 - Nitric oxide and secondary oxides of nitrogen react with unsaturated fatty acids such as linoleic acid to yield oxidized and nitrated products. Fatty acid nitroalkene derivatives, (e.g. nitrolinoleate [LNO 2]) are produced by oxidative inflammatory reactions, detected clinically, display potent electrophilic reactivity and induce post-translational protein modifications that mediate adaptive inflammatory signaling responses. LNO 2 signaling was examined in lung epithelial cells because the alveolar compartment is a rich site for the transduction of redox and inflammatory reactions. LNO 2 did not directly induce Ca 2+ influx in cultured lung epithelial cells, but inhibited bradykinin-induced Ca 2+ influx in a cGMP-independent manner. In contrast, LNO 2 activated MAP kinase (Erk1/2) by a mechanism independent of bradykinin. It was hypothesized that these unique responses were transduced by activation of different protein kinase C isotypes, supported by the observation that LNO 2-mediated inhibition of Ca 2+ influx was blocked by the non-selective PKC inhibitors chelerythine chloride and calphostin C, but not by the calcium dependent "classic" PKC inhibitor Gö6976. Western blot analysis showed that atypical PKCζ was activated by LNO 2 stimulation, with PKCζ and Erk activation also demonstrated in primary culture of human lung type II cells. Addition of pseudotypical PKCζ substrate peptide reversed LNO 2-mediated induction of Ca 2+ influx and MAP kinase activation. Finally, the electrophilic nature of LNO 2 resulted in a novel mode of PKCζ activation, covalent adduction of the enzyme. In summary, LNO 2 mediated signaling in lung type II epithelial cells occurs via a unique pathway involving PKCζ.

AB - Nitric oxide and secondary oxides of nitrogen react with unsaturated fatty acids such as linoleic acid to yield oxidized and nitrated products. Fatty acid nitroalkene derivatives, (e.g. nitrolinoleate [LNO 2]) are produced by oxidative inflammatory reactions, detected clinically, display potent electrophilic reactivity and induce post-translational protein modifications that mediate adaptive inflammatory signaling responses. LNO 2 signaling was examined in lung epithelial cells because the alveolar compartment is a rich site for the transduction of redox and inflammatory reactions. LNO 2 did not directly induce Ca 2+ influx in cultured lung epithelial cells, but inhibited bradykinin-induced Ca 2+ influx in a cGMP-independent manner. In contrast, LNO 2 activated MAP kinase (Erk1/2) by a mechanism independent of bradykinin. It was hypothesized that these unique responses were transduced by activation of different protein kinase C isotypes, supported by the observation that LNO 2-mediated inhibition of Ca 2+ influx was blocked by the non-selective PKC inhibitors chelerythine chloride and calphostin C, but not by the calcium dependent "classic" PKC inhibitor Gö6976. Western blot analysis showed that atypical PKCζ was activated by LNO 2 stimulation, with PKCζ and Erk activation also demonstrated in primary culture of human lung type II cells. Addition of pseudotypical PKCζ substrate peptide reversed LNO 2-mediated induction of Ca 2+ influx and MAP kinase activation. Finally, the electrophilic nature of LNO 2 resulted in a novel mode of PKCζ activation, covalent adduction of the enzyme. In summary, LNO 2 mediated signaling in lung type II epithelial cells occurs via a unique pathway involving PKCζ.

KW - Calcium mobilization

KW - Nitrated lipids

KW - Protein kinase C

KW - Pulmonary epithelial cell

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=80053916796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053916796&partnerID=8YFLogxK

U2 - 10.1016/j.niox.2011.07.003

DO - 10.1016/j.niox.2011.07.003

M3 - Article

C2 - 21871968

AN - SCOPUS:80053916796

VL - 25

SP - 366

EP - 372

JO - Nitric Oxide - Biology and Chemistry

JF - Nitric Oxide - Biology and Chemistry

SN - 1089-8603

IS - 3

ER -