Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages

Valeriya L. Kudryavtseva, Ksenia Sergeevna Stankevich, Alexandu Gudima, Elina Kibler, Yuri Zhukov, Evgeniy Bolbasov, Anna Malashicheva, Mikhail Zhuravlev, Vladimir Riabov, Tengfei Liu, Victor Filimonov, Gennady Remnev, Harald Klüter, Julia Kzhyshkowska, Sergei Tverdokhlebov

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.

Original languageEnglish
Pages (from-to)261-271
Number of pages11
JournalMaterials and Design
Volume127
DOIs
Publication statusPublished - 5 Aug 2017

Fingerprint

Hyaluronic acid
Macrophages
Hyaluronic Acid
Scaffolds (biology)
Tissue engineering
Atmospheric pressure
Scaffolds
Plasmas
Acids
Tissue Scaffolds
poly(lactic acid)
Hydrophilicity
Matrix Metalloproteinases
Biocompatibility
Wetting
Polymers
X ray photoelectron spectroscopy
Chemical activation
Cytokines
Scanning electron microscopy

Keywords

  • Cytokines
  • Hyaluronic acid (HA)
  • Macrophages
  • Plasma
  • Polylactic acid (PLA)
  • Tissue engineering scaffold (TES)

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages. / Kudryavtseva, Valeriya L.; Stankevich, Ksenia Sergeevna; Gudima, Alexandu; Kibler, Elina; Zhukov, Yuri; Bolbasov, Evgeniy; Malashicheva, Anna; Zhuravlev, Mikhail; Riabov, Vladimir; Liu, Tengfei; Filimonov, Victor; Remnev, Gennady; Klüter, Harald; Kzhyshkowska, Julia; Tverdokhlebov, Sergei.

In: Materials and Design, Vol. 127, 05.08.2017, p. 261-271.

Research output: Contribution to journalArticle

@article{8a2ed2dc86884d6aa113831d05ee3687,
title = "Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages",
abstract = "Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.",
keywords = "Cytokines, Hyaluronic acid (HA), Macrophages, Plasma, Polylactic acid (PLA), Tissue engineering scaffold (TES)",
author = "Kudryavtseva, {Valeriya L.} and Ksenia Sergeevna Stankevich and Alexandu Gudima and Elina Kibler and Yuri Zhukov and Evgeniy Bolbasov and Anna Malashicheva and Mikhail Zhuravlev and Vladimir Riabov and Tengfei Liu and Victor Filimonov and Gennady Remnev and Harald Kl{\"u}ter and Julia Kzhyshkowska and Sergei Tverdokhlebov",
year = "2017",
month = "8",
day = "5",
doi = "10.1016/j.matdes.2017.04.079",
language = "English",
volume = "127",
pages = "261--271",
journal = "Materials and Design",
issn = "0261-3069",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages

AU - Kudryavtseva, Valeriya L.

AU - Stankevich, Ksenia Sergeevna

AU - Gudima, Alexandu

AU - Kibler, Elina

AU - Zhukov, Yuri

AU - Bolbasov, Evgeniy

AU - Malashicheva, Anna

AU - Zhuravlev, Mikhail

AU - Riabov, Vladimir

AU - Liu, Tengfei

AU - Filimonov, Victor

AU - Remnev, Gennady

AU - Klüter, Harald

AU - Kzhyshkowska, Julia

AU - Tverdokhlebov, Sergei

PY - 2017/8/5

Y1 - 2017/8/5

N2 - Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.

AB - Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.

KW - Cytokines

KW - Hyaluronic acid (HA)

KW - Macrophages

KW - Plasma

KW - Polylactic acid (PLA)

KW - Tissue engineering scaffold (TES)

UR - http://www.scopus.com/inward/record.url?scp=85018428716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018428716&partnerID=8YFLogxK

U2 - 10.1016/j.matdes.2017.04.079

DO - 10.1016/j.matdes.2017.04.079

M3 - Article

VL - 127

SP - 261

EP - 271

JO - Materials and Design

JF - Materials and Design

SN - 0261-3069

ER -