Abstract
Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.
| Original language | English |
|---|---|
| Pages (from-to) | 261-271 |
| Number of pages | 11 |
| Journal | Materials and Design |
| Volume | 127 |
| DOIs | |
| Publication status | Published - 5 Aug 2017 |
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Keywords
- Cytokines
- Hyaluronic acid (HA)
- Macrophages
- Plasma
- Polylactic acid (PLA)
- Tissue engineering scaffold (TES)
ASJC Scopus subject areas
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering
Cite this
Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages. / Kudryavtseva, Valeriya L.; Stankevich, Ksenia Sergeevna; Gudima, Alexandu; Kibler, Elina; Zhukov, Yuri; Bolbasov, Evgeniy; Malashicheva, Anna; Zhuravlev, Mikhail; Riabov, Vladimir; Liu, Tengfei; Filimonov, Victor; Remnev, Gennady; Klüter, Harald; Kzhyshkowska, Julia; Tverdokhlebov, Sergei.
In: Materials and Design, Vol. 127, 05.08.2017, p. 261-271.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Atmospheric pressure plasma assisted immobilization of hyaluronic acid on tissue engineering PLA-based scaffolds and its effect on primary human macrophages
AU - Kudryavtseva, Valeriya L.
AU - Stankevich, Ksenia Sergeevna
AU - Gudima, Alexandu
AU - Kibler, Elina
AU - Zhukov, Yuri
AU - Bolbasov, Evgeniy
AU - Malashicheva, Anna
AU - Zhuravlev, Mikhail
AU - Riabov, Vladimir
AU - Liu, Tengfei
AU - Filimonov, Victor
AU - Remnev, Gennady
AU - Klüter, Harald
AU - Kzhyshkowska, Julia
AU - Tverdokhlebov, Sergei
PY - 2017/8/5
Y1 - 2017/8/5
N2 - Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.
AB - Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified - plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high-performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.
KW - Cytokines
KW - Hyaluronic acid (HA)
KW - Macrophages
KW - Plasma
KW - Polylactic acid (PLA)
KW - Tissue engineering scaffold (TES)
UR - http://www.scopus.com/inward/record.url?scp=85018428716&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018428716&partnerID=8YFLogxK
U2 - 10.1016/j.matdes.2017.04.079
DO - 10.1016/j.matdes.2017.04.079
M3 - Article
VL - 127
SP - 261
EP - 271
JO - Materials and Design
JF - Materials and Design
SN - 0261-3069
ER -