Abstract
The aim of this work was voltammetric determination of 1-aminopyrene and 1-hydroxypyrene using carbon paste electrodes modified with cyclodextrin derivatives and double stranded deoxyribonucleic acid (dsDNA). The detection schemes based on a preconcentration and differential pulse voltammetric (DPV) determination at β-cyclodextrin and γ-cyclodextrin modified carbon paste electrode (β-CD/CPE, γ-CD/CPE), neutral β-cyclodextrin polymer and carboxymethyl-β-cyclodextrin polymer modified screen-printed electrode (β-CDP/SPE, β-CDPA/SPE) and dsDNA modified screen-printed electrode (DNA/SPE) are proposed for the trace determination of studied analytes within the concentration range from 2×10-8 to 4×10 -7 mol dm-3 and from 2×10-7 to 4×10-6 mol dm-3 with the limits of quantification down to 10-8 mol dm-3. Depending on pH, 1-aminopyrene interacts with both surface attached CD and DNA by electrostatic bonds and supramolecular complexation while 1-hydroxypyrene associates with the CD hosts via complexation. The 1-aminopyrene interaction with dsDNA was confirmed by fluorimetric measurements in the solution phase using a competing DNA-TO-PRO-3 dye complex. In addition, the effect of temperature on this association was investigated using an electrically heated DNA-modified carbon paste electrode (DNA/CPE).
| Original language | English |
|---|---|
| Pages (from-to) | 191-197 |
| Number of pages | 7 |
| Journal | Bioelectrochemistry |
| Volume | 67 |
| Issue number | 2 SPEC. ISS. |
| DOIs | |
| Publication status | Published - 1 Jan 2005 |
| Externally published | Yes |
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Keywords
- Biosensor
- DNA
- Electrically heated electrode
- Pyrene derivatives
- Screen-printed electrode
- β-Cyclodextrin
ASJC Scopus subject areas
- Biophysics
- Physical and Theoretical Chemistry
- Electrochemistry
Cite this
Association interaction and voltammetric determination of 1-aminopyrene and 1-hydroxypyrene at cyclodextrin and DNA based electrochemical sensors. / Ferancová, A.; Bucková, M.; Korgová, E.; Korbut, O.; Gründler, P.; Wärnmark, I.; Štepán, R.; Barek, J.; Zima, J.; Labuda, J.
In: Bioelectrochemistry, Vol. 67, No. 2 SPEC. ISS., 01.01.2005, p. 191-197.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association interaction and voltammetric determination of 1-aminopyrene and 1-hydroxypyrene at cyclodextrin and DNA based electrochemical sensors
AU - Ferancová, A.
AU - Bucková, M.
AU - Korgová, E.
AU - Korbut, O.
AU - Gründler, P.
AU - Wärnmark, I.
AU - Štepán, R.
AU - Barek, J.
AU - Zima, J.
AU - Labuda, J.
PY - 2005/1/1
Y1 - 2005/1/1
N2 - The aim of this work was voltammetric determination of 1-aminopyrene and 1-hydroxypyrene using carbon paste electrodes modified with cyclodextrin derivatives and double stranded deoxyribonucleic acid (dsDNA). The detection schemes based on a preconcentration and differential pulse voltammetric (DPV) determination at β-cyclodextrin and γ-cyclodextrin modified carbon paste electrode (β-CD/CPE, γ-CD/CPE), neutral β-cyclodextrin polymer and carboxymethyl-β-cyclodextrin polymer modified screen-printed electrode (β-CDP/SPE, β-CDPA/SPE) and dsDNA modified screen-printed electrode (DNA/SPE) are proposed for the trace determination of studied analytes within the concentration range from 2×10-8 to 4×10 -7 mol dm-3 and from 2×10-7 to 4×10-6 mol dm-3 with the limits of quantification down to 10-8 mol dm-3. Depending on pH, 1-aminopyrene interacts with both surface attached CD and DNA by electrostatic bonds and supramolecular complexation while 1-hydroxypyrene associates with the CD hosts via complexation. The 1-aminopyrene interaction with dsDNA was confirmed by fluorimetric measurements in the solution phase using a competing DNA-TO-PRO-3 dye complex. In addition, the effect of temperature on this association was investigated using an electrically heated DNA-modified carbon paste electrode (DNA/CPE).
AB - The aim of this work was voltammetric determination of 1-aminopyrene and 1-hydroxypyrene using carbon paste electrodes modified with cyclodextrin derivatives and double stranded deoxyribonucleic acid (dsDNA). The detection schemes based on a preconcentration and differential pulse voltammetric (DPV) determination at β-cyclodextrin and γ-cyclodextrin modified carbon paste electrode (β-CD/CPE, γ-CD/CPE), neutral β-cyclodextrin polymer and carboxymethyl-β-cyclodextrin polymer modified screen-printed electrode (β-CDP/SPE, β-CDPA/SPE) and dsDNA modified screen-printed electrode (DNA/SPE) are proposed for the trace determination of studied analytes within the concentration range from 2×10-8 to 4×10 -7 mol dm-3 and from 2×10-7 to 4×10-6 mol dm-3 with the limits of quantification down to 10-8 mol dm-3. Depending on pH, 1-aminopyrene interacts with both surface attached CD and DNA by electrostatic bonds and supramolecular complexation while 1-hydroxypyrene associates with the CD hosts via complexation. The 1-aminopyrene interaction with dsDNA was confirmed by fluorimetric measurements in the solution phase using a competing DNA-TO-PRO-3 dye complex. In addition, the effect of temperature on this association was investigated using an electrically heated DNA-modified carbon paste electrode (DNA/CPE).
KW - Biosensor
KW - DNA
KW - Electrically heated electrode
KW - Pyrene derivatives
KW - Screen-printed electrode
KW - β-Cyclodextrin
UR - http://www.scopus.com/inward/record.url?scp=23944452373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23944452373&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2004.06.007
DO - 10.1016/j.bioelechem.2004.06.007
M3 - Article
C2 - 16122687
AN - SCOPUS:23944452373
VL - 67
SP - 191
EP - 197
JO - Bioelectrochemistry
JF - Bioelectrochemistry
SN - 1567-5394
IS - 2 SPEC. ISS.
ER -