Association interaction and voltammetric determination of 1-aminopyrene and 1-hydroxypyrene at cyclodextrin and DNA based electrochemical sensors

A. Ferancová, M. Bucková, E. Korgová, O. Korbut, P. Gründler, I. Wärnmark, R. Štepán, J. Barek, J. Zima, J. Labuda

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28 Citations (Scopus)

Abstract

The aim of this work was voltammetric determination of 1-aminopyrene and 1-hydroxypyrene using carbon paste electrodes modified with cyclodextrin derivatives and double stranded deoxyribonucleic acid (dsDNA). The detection schemes based on a preconcentration and differential pulse voltammetric (DPV) determination at β-cyclodextrin and γ-cyclodextrin modified carbon paste electrode (β-CD/CPE, γ-CD/CPE), neutral β-cyclodextrin polymer and carboxymethyl-β-cyclodextrin polymer modified screen-printed electrode (β-CDP/SPE, β-CDPA/SPE) and dsDNA modified screen-printed electrode (DNA/SPE) are proposed for the trace determination of studied analytes within the concentration range from 2×10-8 to 4×10 -7 mol dm-3 and from 2×10-7 to 4×10-6 mol dm-3 with the limits of quantification down to 10-8 mol dm-3. Depending on pH, 1-aminopyrene interacts with both surface attached CD and DNA by electrostatic bonds and supramolecular complexation while 1-hydroxypyrene associates with the CD hosts via complexation. The 1-aminopyrene interaction with dsDNA was confirmed by fluorimetric measurements in the solution phase using a competing DNA-TO-PRO-3 dye complex. In addition, the effect of temperature on this association was investigated using an electrically heated DNA-modified carbon paste electrode (DNA/CPE).

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalBioelectrochemistry
Volume67
Issue number2 SPEC. ISS.
DOIs
Publication statusPublished - 1 Jan 2005
Externally publishedYes

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Keywords

  • Biosensor
  • DNA
  • Electrically heated electrode
  • Pyrene derivatives
  • Screen-printed electrode
  • β-Cyclodextrin

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

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