Abstract
The aim of this work was voltammetric determination of 1-aminopyrene and 1-hydroxypyrene using carbon paste electrodes modified with cyclodextrin derivatives and double stranded deoxyribonucleic acid (dsDNA). The detection schemes based on a preconcentration and differential pulse voltammetric (DPV) determination at β-cyclodextrin and γ-cyclodextrin modified carbon paste electrode (β-CD/CPE, γ-CD/CPE), neutral β-cyclodextrin polymer and carboxymethyl-β-cyclodextrin polymer modified screen-printed electrode (β-CDP/SPE, β-CDPA/SPE) and dsDNA modified screen-printed electrode (DNA/SPE) are proposed for the trace determination of studied analytes within the concentration range from 2×10-8 to 4×10 -7 mol dm-3 and from 2×10-7 to 4×10-6 mol dm-3 with the limits of quantification down to 10-8 mol dm-3. Depending on pH, 1-aminopyrene interacts with both surface attached CD and DNA by electrostatic bonds and supramolecular complexation while 1-hydroxypyrene associates with the CD hosts via complexation. The 1-aminopyrene interaction with dsDNA was confirmed by fluorimetric measurements in the solution phase using a competing DNA-TO-PRO-3 dye complex. In addition, the effect of temperature on this association was investigated using an electrically heated DNA-modified carbon paste electrode (DNA/CPE).
Original language | English |
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Pages (from-to) | 191-197 |
Number of pages | 7 |
Journal | Bioelectrochemistry |
Volume | 67 |
Issue number | 2 SPEC. ISS. |
DOIs | |
Publication status | Published - 1 Jan 2005 |
Externally published | Yes |
Keywords
- Biosensor
- DNA
- Electrically heated electrode
- Pyrene derivatives
- Screen-printed electrode
- β-Cyclodextrin
ASJC Scopus subject areas
- Biophysics
- Physical and Theoretical Chemistry
- Electrochemistry