Aspergillus fumigatus-induced allergic airway inflammation alters surfactant homeostasis and lung function in BALB/c mice

A. Haczku, Elena Nikolaevna Atochina-Vasserman, Y. Tomer, H. Chen, S. T. Scanlon, S. Russo, J. Xu, Jr Panettieri R.A., M. F. Beers

Research output: Contribution to journalArticle

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Abstract

The differential regulation of pulmonary surfactant proteins (5Ps) is demonstrated in a murine model of Aspergillus fumigatus (Af)-induced allergic airway inflammation and hyperresponsiveness. BALB/c mice were sensitized intraperitoneally and challenged intranasally with Af extract. Enzyme-linked immunosorbent assay analysis of serum immunoglobulin (Ig) levels in these mice showed markedly increased total IgE and Af-specific IgE and IgG1. This was associated with peribronchial/perivascular tissue inflammation, airway eosinophilia, and secretion of interleukin (IL)-4 and IL-5 into the bronchoalveolar lavage fluid (BALI:). Functional analysis revealed that in comparison with nonsensitized mice, allergic sensitization and challenge resulted in significant increases in acetylcholine responsiveness. To analyze levels of SPs, the cell-free supernate of the BALF was further fractionated by high-speed (20,000 × g) centrifugation. After sensitization and challenges, the pellet (large-aggregate fraction) showed a selective downregulation of hydrophobic SPs SP-B and SP-C by 50%. This reduction was reflected by commensurate decreases in SP-B and SP-C messenger RNA (mRNA) expression of the lung tissue of these animals. In contrast, there was a 9-fold increase in SP-D protein levels in the 20,000 × g supernate without changes in SP-D mRNA. The increased levels of SP-D showed a significant positive correlation with serum IgE (r = 0.85, P <0.001). Tissue mRNA and protein levels of 5P-A in either the large- or the small-aggregate fractions were unaffected. Our data indicate that allergic airway inflammation induces selective inhibition of hydrophobic SP synthesis accompanied by marked increases in the lung collectin SP-D protein content of BALF. These changes may contribute significantly to the pathophysiology of Af-induced allergic airway hyperresponsiveness.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume25
Issue number1
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein D
Aspergillus fumigatus
Aspergillus
Surface-Active Agents
Homeostasis
Immunoglobulin E
Inflammation
Lung
Tissue
Messenger RNA
Pulmonary Surfactant-Associated Proteins
Collectins
Immunosorbents
Functional analysis
Proteins
Centrifugation
Interleukin-5
Bronchoalveolar Lavage Fluid
Eosinophilia
Serum

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Aspergillus fumigatus-induced allergic airway inflammation alters surfactant homeostasis and lung function in BALB/c mice. / Haczku, A.; Atochina-Vasserman, Elena Nikolaevna; Tomer, Y.; Chen, H.; Scanlon, S. T.; Russo, S.; Xu, J.; Panettieri R.A., Jr; Beers, M. F.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 25, No. 1, 2001, p. 45-50.

Research output: Contribution to journalArticle

Haczku, A. ; Atochina-Vasserman, Elena Nikolaevna ; Tomer, Y. ; Chen, H. ; Scanlon, S. T. ; Russo, S. ; Xu, J. ; Panettieri R.A., Jr ; Beers, M. F. / Aspergillus fumigatus-induced allergic airway inflammation alters surfactant homeostasis and lung function in BALB/c mice. In: American Journal of Respiratory Cell and Molecular Biology. 2001 ; Vol. 25, No. 1. pp. 45-50.
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AU - Atochina-Vasserman, Elena Nikolaevna

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AU - Chen, H.

AU - Scanlon, S. T.

AU - Russo, S.

AU - Xu, J.

AU - Panettieri R.A., Jr

AU - Beers, M. F.

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