Aquaporin 11 insufficiency modulates kidney susceptibility to oxidative stress

Elena N. Atochina-Vasserman, Asel Biktasova, Elena Abramova, Dong Sheng Cheng, Vasiliy V. Polosukhin, Harikrishna Tanjore, Saki Takahashi, Hiroko Sonoda, Liberty Foye, Christo Venkov, Sergey V. Ryzhov, Sergey Novitskiy, Natalia Shlonimskaya, Masahiro Ikeda, Timothy S. Blackwell, William E. Lawson, Andrew J. Gow, Raymond C. Harris, Mikhail M. Dikov, Elena E. Tchekneva

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Aquaporin 11 (AQP11) is a newly described member of the protein family of transport channels. AQP11 associates with the endoplasmic reticulum (ER) and is highly expressed in proximal tubular epithelial cells in the kidney. Previously, we identified and characterized a recessive mutation of the highly conserved Cys227 to Ser227 in mouse AQP11 that caused proximal tubule (PT) injury and kidney failure in mutant mice. The current study revealed induction of ER stress, unfolded protein response, and apoptosis as molecular mechanisms of this PT injury. Cys227Ser mutation interfered with maintenance of AQP11 oligo-meric structure. AQP11 is abundantly expressed in the S1 PT segment, a site of major renal glucose flux, and Aqp11 mutant mice developed PT-specific mitochondrial injury. Glucose increased AQP11 protein expression in wild-type kidney and upregulation of AQP11 expression by glucose in vitro was prevented by phlorizin, an inhibitor of sodium-dependent glucose transport across PT. Total AQP11 levels in heterozygotes were higher than in wild-type mice but were not further increased in response to glucose. In Aqp11 insufficient PT cells, glucose potentiated increases in reactive oxygen species (ROS) production. ROS production was also elevated in Aqp11 mutation carriers. Phenotypically normal mice heterozygous for the Aqp11 mutation repeatedly treated with glucose showed increased blood urea nitrogen levels that were prevented by the antioxidant sulforaphane or by phlorizin. Our results indicate an important role for AQP11 to prevent glucose-induced oxidative stress in proximal tubules.

Original languageEnglish
Pages (from-to)1295-1307
Number of pages13
JournalAmerican Journal of Physiology - Renal Physiology
Volume304
Issue number10
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Aquaporins
Renal Insufficiency
Oxidative Stress
Glucose
Phlorhizin
Mutation
Kidney
Reactive Oxygen Species
Wounds and Injuries
Unfolded Protein Response
Endoplasmic Reticulum Stress
Blood Urea Nitrogen
Protein Transport
Heterozygote
Heat-Shock Proteins
Endoplasmic Reticulum
Up-Regulation
Antioxidants
Epithelial Cells
Sodium

Keywords

  • Acute kidney injury
  • Protein oligomerization
  • Proximal tubules

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Atochina-Vasserman, E. N., Biktasova, A., Abramova, E., Cheng, D. S., Polosukhin, V. V., Tanjore, H., ... Tchekneva, E. E. (2013). Aquaporin 11 insufficiency modulates kidney susceptibility to oxidative stress. American Journal of Physiology - Renal Physiology, 304(10), 1295-1307. https://doi.org/10.1152/ajprenal.00344.2012

Aquaporin 11 insufficiency modulates kidney susceptibility to oxidative stress. / Atochina-Vasserman, Elena N.; Biktasova, Asel; Abramova, Elena; Cheng, Dong Sheng; Polosukhin, Vasiliy V.; Tanjore, Harikrishna; Takahashi, Saki; Sonoda, Hiroko; Foye, Liberty; Venkov, Christo; Ryzhov, Sergey V.; Novitskiy, Sergey; Shlonimskaya, Natalia; Ikeda, Masahiro; Blackwell, Timothy S.; Lawson, William E.; Gow, Andrew J.; Harris, Raymond C.; Dikov, Mikhail M.; Tchekneva, Elena E.

In: American Journal of Physiology - Renal Physiology, Vol. 304, No. 10, 2013, p. 1295-1307.

Research output: Contribution to journalArticle

Atochina-Vasserman, EN, Biktasova, A, Abramova, E, Cheng, DS, Polosukhin, VV, Tanjore, H, Takahashi, S, Sonoda, H, Foye, L, Venkov, C, Ryzhov, SV, Novitskiy, S, Shlonimskaya, N, Ikeda, M, Blackwell, TS, Lawson, WE, Gow, AJ, Harris, RC, Dikov, MM & Tchekneva, EE 2013, 'Aquaporin 11 insufficiency modulates kidney susceptibility to oxidative stress', American Journal of Physiology - Renal Physiology, vol. 304, no. 10, pp. 1295-1307. https://doi.org/10.1152/ajprenal.00344.2012
Atochina-Vasserman, Elena N. ; Biktasova, Asel ; Abramova, Elena ; Cheng, Dong Sheng ; Polosukhin, Vasiliy V. ; Tanjore, Harikrishna ; Takahashi, Saki ; Sonoda, Hiroko ; Foye, Liberty ; Venkov, Christo ; Ryzhov, Sergey V. ; Novitskiy, Sergey ; Shlonimskaya, Natalia ; Ikeda, Masahiro ; Blackwell, Timothy S. ; Lawson, William E. ; Gow, Andrew J. ; Harris, Raymond C. ; Dikov, Mikhail M. ; Tchekneva, Elena E. / Aquaporin 11 insufficiency modulates kidney susceptibility to oxidative stress. In: American Journal of Physiology - Renal Physiology. 2013 ; Vol. 304, No. 10. pp. 1295-1307.
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AU - Cheng, Dong Sheng

AU - Polosukhin, Vasiliy V.

AU - Tanjore, Harikrishna

AU - Takahashi, Saki

AU - Sonoda, Hiroko

AU - Foye, Liberty

AU - Venkov, Christo

AU - Ryzhov, Sergey V.

AU - Novitskiy, Sergey

AU - Shlonimskaya, Natalia

AU - Ikeda, Masahiro

AU - Blackwell, Timothy S.

AU - Lawson, William E.

AU - Gow, Andrew J.

AU - Harris, Raymond C.

AU - Dikov, Mikhail M.

AU - Tchekneva, Elena E.

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KW - Acute kidney injury

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