Application of hollow fibre based microextraction for voltammetric determination of vanillylmandelic acid in human urine

Vojtěch Hrdlička, Tomáš Navrátil, Jiří Barek

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5 Citations (Scopus)


A new hollow-fibre microextraction method has been developed for the determination of a clinical biomarker vanillylmandelic acid (VMA) in human urine. The extraction was used in combination with differential pulse voltammetry (DPV) on cathodically pre-treated boron doped diamond electrode (BDDE). Butyl benzoate was found to be the optimum solvent for creation of supported liquid membrane. Optimum donor and acceptor phases were 0.1 mol L −1 HCl and 0.1 mol L −1 NaOH, respectively, optimum extraction time was 30 min. Linear range was from 0.5 to 100 μmol L −1 (r = 0.9989), with good repeatability (RSD 7.9% for 50 μmol L −1 VMA aqueous sample (n = 7)). The limit of detection (LOD) was 0.5 μmol L −1 . The method was successfully applied for the determination of VMA in real human urine sample using a standard addition method, obtained RSD being 8.1% (n = 5). The method is sufficiently selective and sensitive for its intended use as a screening tool for elevated concentration of VMA.

Original languageEnglish
Pages (from-to)130-136
Number of pages7
JournalJournal of Electroanalytical Chemistry
Publication statusPublished - 15 Feb 2019
Externally publishedYes



  • Biomarker
  • Boron doped diamond electrode (BDDE)
  • Hollow fibre
  • Liquid phase microextraction
  • Vanillylmandelic acid (VMA)
  • Voltammetry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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