Abstract
Dependance of antioxidant activity of the humic macromolecular compounds from their etiology and production method was learned by means of physical-chemical analysis methods - colorimetry with diphenylpicrylhydrazyl (DPPH) as a stable radical, cathodic voltammetry, electron paramagnetic resonance spectroscopy. 18 native humic acids (HAs) were isolated by extraction from nine typical species of peats of the Tomsk region, that differ from each other by botanical composition, degree of decomposition and ash content. Two extragents were used: sodium hydroxide and sodium pyrophosphate. Although all 18 HAs samples exhibit antioxidant activity. At the same time there is a similar trend in the distribution of antioxidant activity, which is proved by Spearman's correlation analysis. A higher activity was noted for pyrophosphate HAs. Using methods of cathodic voltammetry and colorimetry with DPPH it was found that HAs show antioxidant activity by its mechanism, being a proton donor due to the presence of a large number of phenolic hydroxyls. EPR spectroscopy established that the HAs are traps of free radicals.
| Original language | English |
|---|---|
| Pages (from-to) | 239-250 |
| Number of pages | 12 |
| Journal | Khimiya Rastitel'nogo Syr'ya |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
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Keywords
- Antioxidants
- Colorimetry
- Diphenylpicrylhydrazyl
- Free radicals
- Humic acids
- Peat
- Spectroscopy
- Voltammetry
ASJC Scopus subject areas
- Biomaterials
- Plant Science
- Organic Chemistry
Cite this
Antioxidant activity of macromolecular compounds of humic etiology. / Zykova, M. V.; Logvinova, L. A.; Krivoshchekov, S. V.; Voronova, O. A.; Lasukova, T. V.; Bratishko, K. A.; Zholobova, G. A.; Golubina, O. A.; Perederina, I. A.; Drygunova, L. A.; Tveryakova, E. N.; Belousov, M. V.
In: Khimiya Rastitel'nogo Syr'ya, No. 3, 01.01.2018, p. 239-250.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antioxidant activity of macromolecular compounds of humic etiology
AU - Zykova, M. V.
AU - Logvinova, L. A.
AU - Krivoshchekov, S. V.
AU - Voronova, O. A.
AU - Lasukova, T. V.
AU - Bratishko, K. A.
AU - Zholobova, G. A.
AU - Golubina, O. A.
AU - Perederina, I. A.
AU - Drygunova, L. A.
AU - Tveryakova, E. N.
AU - Belousov, M. V.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Dependance of antioxidant activity of the humic macromolecular compounds from their etiology and production method was learned by means of physical-chemical analysis methods - colorimetry with diphenylpicrylhydrazyl (DPPH) as a stable radical, cathodic voltammetry, electron paramagnetic resonance spectroscopy. 18 native humic acids (HAs) were isolated by extraction from nine typical species of peats of the Tomsk region, that differ from each other by botanical composition, degree of decomposition and ash content. Two extragents were used: sodium hydroxide and sodium pyrophosphate. Although all 18 HAs samples exhibit antioxidant activity. At the same time there is a similar trend in the distribution of antioxidant activity, which is proved by Spearman's correlation analysis. A higher activity was noted for pyrophosphate HAs. Using methods of cathodic voltammetry and colorimetry with DPPH it was found that HAs show antioxidant activity by its mechanism, being a proton donor due to the presence of a large number of phenolic hydroxyls. EPR spectroscopy established that the HAs are traps of free radicals.
AB - Dependance of antioxidant activity of the humic macromolecular compounds from their etiology and production method was learned by means of physical-chemical analysis methods - colorimetry with diphenylpicrylhydrazyl (DPPH) as a stable radical, cathodic voltammetry, electron paramagnetic resonance spectroscopy. 18 native humic acids (HAs) were isolated by extraction from nine typical species of peats of the Tomsk region, that differ from each other by botanical composition, degree of decomposition and ash content. Two extragents were used: sodium hydroxide and sodium pyrophosphate. Although all 18 HAs samples exhibit antioxidant activity. At the same time there is a similar trend in the distribution of antioxidant activity, which is proved by Spearman's correlation analysis. A higher activity was noted for pyrophosphate HAs. Using methods of cathodic voltammetry and colorimetry with DPPH it was found that HAs show antioxidant activity by its mechanism, being a proton donor due to the presence of a large number of phenolic hydroxyls. EPR spectroscopy established that the HAs are traps of free radicals.
KW - Antioxidants
KW - Colorimetry
KW - Diphenylpicrylhydrazyl
KW - Free radicals
KW - Humic acids
KW - Peat
KW - Spectroscopy
KW - Voltammetry
UR - http://www.scopus.com/inward/record.url?scp=85054182897&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054182897&partnerID=8YFLogxK
U2 - 10.14258/jcprm.2018033925
DO - 10.14258/jcprm.2018033925
M3 - Article
AN - SCOPUS:85054182897
SP - 239
EP - 250
JO - Khimiya Rastitel'nogo Syr'ya
JF - Khimiya Rastitel'nogo Syr'ya
SN - 1029-5151
IS - 3
ER -