Antioxidant activity of humic substances via bioluminescent monitoring in vitro

A. S. Tarasova, D. I. Stom, N. S. Kudryasheva

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


This work considers antioxidant properties of natural detoxifying agents—humic substances (HS) in solutions of model inorganic and organic compounds of oxidative nature—complex salt K3[Fe(СN)6] and 1,4-benzoquinone. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay in vitro to monitor toxicity of the oxidizer solutions. Toxicities of general and oxidative types were evaluated using bioluminescent kinetic parameters—bioluminescence intensity and induction period, respectively. Antioxidant activity of HS was attributed to their ability to decrease both general and oxidative toxicities; the HS antioxidant efficiency was characterized with detoxification coefficients DGT and DOxT, respectively. Dependencies of DGT and DOxT on HS concentration and time of preliminary incubation of the oxidizers with HS were demonstrated. The optimal conditions for detoxification of the oxidizers were >20-min incubation time and 0.5 × 10−4 to 2 × 10−4 M of HS concentration. The present study promotes application of the enzymatic luminescent bioassay to monitor toxicity of pollutants of oxidative nature in environmental and waste waters in remediation procedures.

Original languageEnglish
JournalEnvironmental Monitoring and Assessment
Issue number3
Publication statusPublished - 2015
Externally publishedYes


  • Antioxidant activity
  • Bioassay
  • Bioluminescence
  • General toxicity
  • Humic substances
  • Oxidative toxicity

ASJC Scopus subject areas

  • Environmental Science(all)
  • Management, Monitoring, Policy and Law
  • Pollution
  • Medicine(all)

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