Anti-HER2 phototoxin based on flavoprotein miniSOG causes the oxidative stress and necrosis of HER2-positive cancer cells

O. N. Shilova, G. M. Proshkina, A. V. Ryabova, S. M. Deyev

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Development and functional characterization of novel, high-affinity protein compounds able to selectively kill human cancer cells is an urgent task of modern biomedical research. In this work, we studied the cytotoxicity of a recombinant phototoxic protein DARPin-miniSOG against the HER2-positive human breast adenocarcinoma cells. It was found that targeted phototoxin DARPin-miniSOG interacts specifically with HER2 receptor and causes the light-induced death of HER2-positive cells by the mechanism of necrosis. Irradiation of the cells in the presence of ascorbic acid eliminates the light-induced cytotoxicity of DARPin-miniSOG, which proves the prooxidant mechanism of phototoxin action.

Original languageEnglish
Pages (from-to)14-18
Number of pages5
JournalMoscow University Biological Sciences Bulletin
Volume71
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Fingerprint

phototoxins
flavoproteins
Flavoproteins
Oxidative stress
cancer
necrosis
Oxidative Stress
Necrosis
oxidative stress
Cells
Cytotoxicity
protein
cytotoxicity
ascorbic acid
Neoplasms
irradiation
biomedical research
cells
adenocarcinoma
Recombinant Proteins

Keywords

  • DNA hypoploid fraction
  • HER2 receptor
  • oxidative stress
  • targeted phototoxin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)

Cite this

Anti-HER2 phototoxin based on flavoprotein miniSOG causes the oxidative stress and necrosis of HER2-positive cancer cells. / Shilova, O. N.; Proshkina, G. M.; Ryabova, A. V.; Deyev, S. M.

In: Moscow University Biological Sciences Bulletin, Vol. 71, No. 1, 01.01.2016, p. 14-18.

Research output: Contribution to journalArticle

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