TY - JOUR
T1 - Analysis of Mechanisms of Interaction between Ciprofloxacin-Based Radiopharmaceutical Labeled with Technetium-99m and Cultured Staphylococcus aureus
AU - Sazonova, Svetlana Ivanovna
AU - Karpova, M. R.
AU - Il’yushenkova, Yu N.
AU - Varlamova, Natalia Valerievna
AU - Lishmanov, Yu B.
PY - 2014/10/31
Y1 - 2014/10/31
N2 - Mechanisms of interaction between ciprofloxacin-based radiopharmaceutical labeled with technetium-99m and cultured Staphylococcus aureus were studied. The substance, reagent for radiopharmaceutical, and the radiopharmaceutical demonstrated similar bactericidal activity against the test bacterial cultures, which confi rmed specifi c antibacterial activity of technetium-99m-labeled ciprofloxacin. The technetium-99m-labeled antibiotic reversibly interacts with inactivated Staphylococcus aureus culture, which indicates the existence of an additional non-specific pathway for radiopharmaceutical accumulation in the bacterial inflammation focus. Our experiment ruled out the possibility of binding free technetium-99m at concentrations <5% (its concentration in the radiopharmaceutical) with Staphylococcus aureus.
AB - Mechanisms of interaction between ciprofloxacin-based radiopharmaceutical labeled with technetium-99m and cultured Staphylococcus aureus were studied. The substance, reagent for radiopharmaceutical, and the radiopharmaceutical demonstrated similar bactericidal activity against the test bacterial cultures, which confi rmed specifi c antibacterial activity of technetium-99m-labeled ciprofloxacin. The technetium-99m-labeled antibiotic reversibly interacts with inactivated Staphylococcus aureus culture, which indicates the existence of an additional non-specific pathway for radiopharmaceutical accumulation in the bacterial inflammation focus. Our experiment ruled out the possibility of binding free technetium-99m at concentrations <5% (its concentration in the radiopharmaceutical) with Staphylococcus aureus.
KW - ciprofloxacin
KW - inflammation
KW - technetium-99m
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U2 - 10.1007/s10517-014-2654-2
DO - 10.1007/s10517-014-2654-2
M3 - Article
C2 - 25342481
AN - SCOPUS:84922083297
VL - 157
SP - 732
EP - 734
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
IS - 6
ER -