Abstract
Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100 % for dopamine, 32.1 % for L-DOPA, 26.2 % for noradrenaline, and 0.71 % for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82–5.18 %) and sensitivity (5.46–15.5 μmol L−1). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
| Original language | English |
|---|---|
| Pages (from-to) | 1163-1171 |
| Number of pages | 9 |
| Journal | Electroanalysis |
| Volume | 30 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Jun 2018 |
| Externally published | Yes |
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Keywords
- Amperometry
- Catecholamines
- Enzymatic biosensor
- Flow analysis
- Silver amalgam
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems. / Josypčuk, Oksana; Barek, Jiří; Josypčuk, Bohdan.
In: Electroanalysis, Vol. 30, No. 6, 01.06.2018, p. 1163-1171.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems
AU - Josypčuk, Oksana
AU - Barek, Jiří
AU - Josypčuk, Bohdan
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100 % for dopamine, 32.1 % for L-DOPA, 26.2 % for noradrenaline, and 0.71 % for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82–5.18 %) and sensitivity (5.46–15.5 μmol L−1). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
AB - Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100 % for dopamine, 32.1 % for L-DOPA, 26.2 % for noradrenaline, and 0.71 % for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82–5.18 %) and sensitivity (5.46–15.5 μmol L−1). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
KW - Amperometry
KW - Catecholamines
KW - Enzymatic biosensor
KW - Flow analysis
KW - Silver amalgam
UR - http://www.scopus.com/inward/record.url?scp=85043703846&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85043703846&partnerID=8YFLogxK
U2 - 10.1002/elan.201800078
DO - 10.1002/elan.201800078
M3 - Article
VL - 30
SP - 1163
EP - 1171
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 6
ER -