Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems

Oksana Josypčuk, Jiří Barek, Bohdan Josypčuk

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100 % for dopamine, 32.1 % for L-DOPA, 26.2 % for noradrenaline, and 0.71 % for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82–5.18 %) and sensitivity (5.46–15.5 μmol L−1). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.

Original languageEnglish
Pages (from-to)1163-1171
Number of pages9
JournalElectroanalysis
Volume30
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018
Externally publishedYes

Keywords

  • Amperometry
  • Catecholamines
  • Enzymatic biosensor
  • Flow analysis
  • Silver amalgam

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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