Amperometric determination of aminobiphenyls using HPLC-ED with boron-doped diamond electrode

Karolina Pecková, Kateřina Jandová, Lucie Maixnerová, Greg M. Swain, Jiří Barek

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Amino derivatives of biphenyl have been proven to be genotoxic and/or carcinogenic, thus they are widely monitored in the environment as well as in biological liquids. This study was devoted to HPLC determination of 2-aminobiphenyl, 3-aminobiphenyl, and 4-aminobiphenyl in model drinking and river water samples using amperometric detection in a thin layer cell with boron doped diamond thin film electrode. Satisfactory separation of studied analytes was achieved at a ChiraDex column (Merck, Germany) with chemically bonded β-cyclodextrin in a mobile phase aqueous buffer/acetonitrile/methanol (40/30/30, v/v/v) with total separation time of six minutes. The highest detector response with satisfactory repeatability (relative standard deviation < 2.9% for all aminobiphenyls) was obtained using 0.01 mol L-1 acetate buffer at pH 5.0 as an aqueous component of the mobile phase. For direct determination of aminobiphenyls in drinking and river water model samples limits of quantitation ca. 4 × 10-7 mol L-1 were achieved. Applying off-line solid phase extraction using Lichrolut EN (Merck, Germany) cartridges and elution by diethyl ether, limits of quantitation were lowered down to the 10-9 mol L-1 concentration range.

Original languageEnglish
Pages (from-to)316-324
Number of pages9
JournalElectroanalysis
Volume21
Issue number3-5
DOIs
Publication statusPublished - 1 Feb 2009
Externally publishedYes

Fingerprint

Diamond
Boron
Diamonds
Buffers
Rivers
Electrodes
Water
Diamond films
Cyclodextrins
Acetonitrile
Ether
Methanol
Ethers
Acetates
Derivatives
Detectors
Thin films
Liquids
3-aminobiphenyl
acetonitrile

Keywords

  • Aminobiphenyls
  • Amperometric detection
  • Boron doped diamond thin film electrode
  • Solid phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Amperometric determination of aminobiphenyls using HPLC-ED with boron-doped diamond electrode. / Pecková, Karolina; Jandová, Kateřina; Maixnerová, Lucie; Swain, Greg M.; Barek, Jiří.

In: Electroanalysis, Vol. 21, No. 3-5, 01.02.2009, p. 316-324.

Research output: Contribution to journalArticle

Pecková, Karolina ; Jandová, Kateřina ; Maixnerová, Lucie ; Swain, Greg M. ; Barek, Jiří. / Amperometric determination of aminobiphenyls using HPLC-ED with boron-doped diamond electrode. In: Electroanalysis. 2009 ; Vol. 21, No. 3-5. pp. 316-324.
@article{fb2b7f14888241cca631f2a12c12667e,
title = "Amperometric determination of aminobiphenyls using HPLC-ED with boron-doped diamond electrode",
abstract = "Amino derivatives of biphenyl have been proven to be genotoxic and/or carcinogenic, thus they are widely monitored in the environment as well as in biological liquids. This study was devoted to HPLC determination of 2-aminobiphenyl, 3-aminobiphenyl, and 4-aminobiphenyl in model drinking and river water samples using amperometric detection in a thin layer cell with boron doped diamond thin film electrode. Satisfactory separation of studied analytes was achieved at a ChiraDex column (Merck, Germany) with chemically bonded β-cyclodextrin in a mobile phase aqueous buffer/acetonitrile/methanol (40/30/30, v/v/v) with total separation time of six minutes. The highest detector response with satisfactory repeatability (relative standard deviation < 2.9{\%} for all aminobiphenyls) was obtained using 0.01 mol L-1 acetate buffer at pH 5.0 as an aqueous component of the mobile phase. For direct determination of aminobiphenyls in drinking and river water model samples limits of quantitation ca. 4 × 10-7 mol L-1 were achieved. Applying off-line solid phase extraction using Lichrolut EN (Merck, Germany) cartridges and elution by diethyl ether, limits of quantitation were lowered down to the 10-9 mol L-1 concentration range.",
keywords = "Aminobiphenyls, Amperometric detection, Boron doped diamond thin film electrode, Solid phase extraction",
author = "Karolina Peckov{\'a} and Kateřina Jandov{\'a} and Lucie Maixnerov{\'a} and Swain, {Greg M.} and Jiř{\'i} Barek",
year = "2009",
month = "2",
day = "1",
doi = "10.1002/elan.200804387",
language = "English",
volume = "21",
pages = "316--324",
journal = "Macromolecular Chemistry and Physics",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "3-5",

}

TY - JOUR

T1 - Amperometric determination of aminobiphenyls using HPLC-ED with boron-doped diamond electrode

AU - Pecková, Karolina

AU - Jandová, Kateřina

AU - Maixnerová, Lucie

AU - Swain, Greg M.

AU - Barek, Jiří

PY - 2009/2/1

Y1 - 2009/2/1

N2 - Amino derivatives of biphenyl have been proven to be genotoxic and/or carcinogenic, thus they are widely monitored in the environment as well as in biological liquids. This study was devoted to HPLC determination of 2-aminobiphenyl, 3-aminobiphenyl, and 4-aminobiphenyl in model drinking and river water samples using amperometric detection in a thin layer cell with boron doped diamond thin film electrode. Satisfactory separation of studied analytes was achieved at a ChiraDex column (Merck, Germany) with chemically bonded β-cyclodextrin in a mobile phase aqueous buffer/acetonitrile/methanol (40/30/30, v/v/v) with total separation time of six minutes. The highest detector response with satisfactory repeatability (relative standard deviation < 2.9% for all aminobiphenyls) was obtained using 0.01 mol L-1 acetate buffer at pH 5.0 as an aqueous component of the mobile phase. For direct determination of aminobiphenyls in drinking and river water model samples limits of quantitation ca. 4 × 10-7 mol L-1 were achieved. Applying off-line solid phase extraction using Lichrolut EN (Merck, Germany) cartridges and elution by diethyl ether, limits of quantitation were lowered down to the 10-9 mol L-1 concentration range.

AB - Amino derivatives of biphenyl have been proven to be genotoxic and/or carcinogenic, thus they are widely monitored in the environment as well as in biological liquids. This study was devoted to HPLC determination of 2-aminobiphenyl, 3-aminobiphenyl, and 4-aminobiphenyl in model drinking and river water samples using amperometric detection in a thin layer cell with boron doped diamond thin film electrode. Satisfactory separation of studied analytes was achieved at a ChiraDex column (Merck, Germany) with chemically bonded β-cyclodextrin in a mobile phase aqueous buffer/acetonitrile/methanol (40/30/30, v/v/v) with total separation time of six minutes. The highest detector response with satisfactory repeatability (relative standard deviation < 2.9% for all aminobiphenyls) was obtained using 0.01 mol L-1 acetate buffer at pH 5.0 as an aqueous component of the mobile phase. For direct determination of aminobiphenyls in drinking and river water model samples limits of quantitation ca. 4 × 10-7 mol L-1 were achieved. Applying off-line solid phase extraction using Lichrolut EN (Merck, Germany) cartridges and elution by diethyl ether, limits of quantitation were lowered down to the 10-9 mol L-1 concentration range.

KW - Aminobiphenyls

KW - Amperometric detection

KW - Boron doped diamond thin film electrode

KW - Solid phase extraction

UR - http://www.scopus.com/inward/record.url?scp=60549104464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60549104464&partnerID=8YFLogxK

U2 - 10.1002/elan.200804387

DO - 10.1002/elan.200804387

M3 - Article

VL - 21

SP - 316

EP - 324

JO - Macromolecular Chemistry and Physics

JF - Macromolecular Chemistry and Physics

SN - 1040-0397

IS - 3-5

ER -