Abstract
Amino derivatives of biphenyl have been proven to be genotoxic and/or carcinogenic, thus they are widely monitored in the environment as well as in biological liquids. This study was devoted to HPLC determination of 2-aminobiphenyl, 3-aminobiphenyl, and 4-aminobiphenyl in model drinking and river water samples using amperometric detection in a thin layer cell with boron doped diamond thin film electrode. Satisfactory separation of studied analytes was achieved at a ChiraDex column (Merck, Germany) with chemically bonded β-cyclodextrin in a mobile phase aqueous buffer/acetonitrile/methanol (40/30/30, v/v/v) with total separation time of six minutes. The highest detector response with satisfactory repeatability (relative standard deviation < 2.9% for all aminobiphenyls) was obtained using 0.01 mol L-1 acetate buffer at pH 5.0 as an aqueous component of the mobile phase. For direct determination of aminobiphenyls in drinking and river water model samples limits of quantitation ca. 4 × 10-7 mol L-1 were achieved. Applying off-line solid phase extraction using Lichrolut EN (Merck, Germany) cartridges and elution by diethyl ether, limits of quantitation were lowered down to the 10-9 mol L-1 concentration range.
| Original language | English |
|---|---|
| Pages (from-to) | 316-324 |
| Number of pages | 9 |
| Journal | Electroanalysis |
| Volume | 21 |
| Issue number | 3-5 |
| DOIs | |
| Publication status | Published - 1 Feb 2009 |
| Externally published | Yes |
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Keywords
- Aminobiphenyls
- Amperometric detection
- Boron doped diamond thin film electrode
- Solid phase extraction
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Amperometric determination of aminobiphenyls using HPLC-ED with boron-doped diamond electrode. / Pecková, Karolina; Jandová, Kateřina; Maixnerová, Lucie; Swain, Greg M.; Barek, Jiří.
In: Electroanalysis, Vol. 21, No. 3-5, 01.02.2009, p. 316-324.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Amperometric determination of aminobiphenyls using HPLC-ED with boron-doped diamond electrode
AU - Pecková, Karolina
AU - Jandová, Kateřina
AU - Maixnerová, Lucie
AU - Swain, Greg M.
AU - Barek, Jiří
PY - 2009/2/1
Y1 - 2009/2/1
N2 - Amino derivatives of biphenyl have been proven to be genotoxic and/or carcinogenic, thus they are widely monitored in the environment as well as in biological liquids. This study was devoted to HPLC determination of 2-aminobiphenyl, 3-aminobiphenyl, and 4-aminobiphenyl in model drinking and river water samples using amperometric detection in a thin layer cell with boron doped diamond thin film electrode. Satisfactory separation of studied analytes was achieved at a ChiraDex column (Merck, Germany) with chemically bonded β-cyclodextrin in a mobile phase aqueous buffer/acetonitrile/methanol (40/30/30, v/v/v) with total separation time of six minutes. The highest detector response with satisfactory repeatability (relative standard deviation < 2.9% for all aminobiphenyls) was obtained using 0.01 mol L-1 acetate buffer at pH 5.0 as an aqueous component of the mobile phase. For direct determination of aminobiphenyls in drinking and river water model samples limits of quantitation ca. 4 × 10-7 mol L-1 were achieved. Applying off-line solid phase extraction using Lichrolut EN (Merck, Germany) cartridges and elution by diethyl ether, limits of quantitation were lowered down to the 10-9 mol L-1 concentration range.
AB - Amino derivatives of biphenyl have been proven to be genotoxic and/or carcinogenic, thus they are widely monitored in the environment as well as in biological liquids. This study was devoted to HPLC determination of 2-aminobiphenyl, 3-aminobiphenyl, and 4-aminobiphenyl in model drinking and river water samples using amperometric detection in a thin layer cell with boron doped diamond thin film electrode. Satisfactory separation of studied analytes was achieved at a ChiraDex column (Merck, Germany) with chemically bonded β-cyclodextrin in a mobile phase aqueous buffer/acetonitrile/methanol (40/30/30, v/v/v) with total separation time of six minutes. The highest detector response with satisfactory repeatability (relative standard deviation < 2.9% for all aminobiphenyls) was obtained using 0.01 mol L-1 acetate buffer at pH 5.0 as an aqueous component of the mobile phase. For direct determination of aminobiphenyls in drinking and river water model samples limits of quantitation ca. 4 × 10-7 mol L-1 were achieved. Applying off-line solid phase extraction using Lichrolut EN (Merck, Germany) cartridges and elution by diethyl ether, limits of quantitation were lowered down to the 10-9 mol L-1 concentration range.
KW - Aminobiphenyls
KW - Amperometric detection
KW - Boron doped diamond thin film electrode
KW - Solid phase extraction
UR - http://www.scopus.com/inward/record.url?scp=60549104464&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60549104464&partnerID=8YFLogxK
U2 - 10.1002/elan.200804387
DO - 10.1002/elan.200804387
M3 - Article
AN - SCOPUS:60549104464
VL - 21
SP - 316
EP - 324
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 3-5
ER -