Allelic variants of rearranged immunoglobulin heavy and light chain genes in hybridoma PTF-02 and parent myeloma

S. M. Deyev, D. N. Urakov, A. G. Stepchenko, O. L. Polanovsky

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The hybridoma PTF-02 secretes an antibody against pig transferrin. Rearranged genes for heavy and light immunoglobulin chains have been studied in the genomes of this hybridoma and in the parent myeloma P3-X63.Ag8.653. The hybridoma was shown to contain three rearranged allelic variants of the heavy chain gene's locus. The gene H2, responsible for synthesis of the heavy chain of the antibody to transferrin, was transmitted in the hybridoma cell from a lymphocyte. Two other genes (H1 and H3) were found both in the hybridoma and parent myeloma genomes. The gene H1 was identified in MOPC21 myeloma, which is a precursor of the X63.Ag8 descendent line. Rearranged k genes were also identified both in the hybridoma and parent myeloma. A functional (K2) gene and a fetal (F) gene appeared in the hybridoma genome from an antigen-stimulated normal lymphocyte. The fetal gene was lost in the course of continuous cultivation of the hybridoma PTF-02 cell line. The gene K1 was transmitted from the myeloma used for fusion. In such a way, the pedigree of rearranged heavy and light chain genes in the hybridoma PTF-02 was established. The results obtained in this work may be relevant to many hybridomas whose immortalizing fusion partner is a MOPC21 derivative, and allow one to identify and isolate functional variable genes to create recombinant constructions.

    Original languageEnglish
    Pages (from-to)45-51
    Number of pages7
    JournalGenetica
    Volume85
    Issue number1
    DOIs
    Publication statusPublished - Jan 1991

    Fingerprint

    Immunoglobulin Light Chain Genes
    immunoglobulin light chains
    immunoglobulin heavy chains
    Immunoglobulin Heavy Chain Genes
    myeloma
    hybridomas
    Hybridomas
    Genes
    genes
    Genome
    Transferrin
    transferrin
    genome
    lymphocytes
    Lymphocytes
    Antibodies
    antibodies
    Pedigree

    Keywords

    • allele's pedigree
    • Hybridoma
    • Immunoglobulin genes

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Genetics
    • Plant Science
    • Insect Science

    Cite this

    Allelic variants of rearranged immunoglobulin heavy and light chain genes in hybridoma PTF-02 and parent myeloma. / Deyev, S. M.; Urakov, D. N.; Stepchenko, A. G.; Polanovsky, O. L.

    In: Genetica, Vol. 85, No. 1, 01.1991, p. 45-51.

    Research output: Contribution to journalArticle

    Deyev, S. M. ; Urakov, D. N. ; Stepchenko, A. G. ; Polanovsky, O. L. / Allelic variants of rearranged immunoglobulin heavy and light chain genes in hybridoma PTF-02 and parent myeloma. In: Genetica. 1991 ; Vol. 85, No. 1. pp. 45-51.
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