Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

Angela M. Groves, Andrew J. Gow, Christopher B. Massa, Le Roy Hall, Jeffrey D. Laskin, Debra L. Laskin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In these studies we determined whether progressive pulmonary inflammation associated with aging in surfactant protein D (Sftpd)-/- mice leads to an exacerbated response to ozone. In Sftpd^/^ mice, but not wild-type (WT) mice, age-related increases in numbers of enlarged vacuolated macrophages were observed in the lung, along with alveolar wall rupture, type 2 cell hyperplasia, and increased bronchoalveolar lavage protein and cell content. Numbers of heme oxygenase + macrophages also increased with age in Sftpd-/- mice, together with classically (iNOS +) and alternatively (mannosereceptor+, YM-1 +, orgalectin-3 +) activated macrophages. In both WT and Sftpd-/- mice, increasing age from 8 to 27 wk was associated with reduced lung stiffness, as reflected by decreases in resistance and elastance spectra; however, this response was reversed in 80-wk-old Sftpd-/- mice. Ozone exposure (0.8 ppm, 3 h) caused increases in lung pathology, alveolar epithelial barrier dysfunction, and numbers of iNOS+ macrophages in 8- and 27-wk-old Sftpd-/-, but not WT mice at 72 h postexposure. Conversely, increases in alternatively activated macrophages were observed in 8-wk-old WT mice following ozone exposure, but not in Sftpd-/- mice. Ozone also caused alterations in both airway and tissue mechanics in Sftpd-/- mice at 8 and 27 wk, but not at 80 wk. These data demonstrate that mild to moderate pulmonary inflammation results in increased sensitivity to ozone; however, in senescent mice, these responses are overwhelmed by the larger effects of age-related increases in baseline inflammation and lung injury.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume305
Issue number8
DOIs
Publication statusPublished - 15 Oct 2013
Externally publishedYes

Fingerprint

Ozone
Lung Injury
Pulmonary Surfactant-Associated Protein D
Mechanics
Pneumonia
Macrophages
Lung
Heme Oxygenase (Decyclizing)
Bronchoalveolar Lavage
Hyperplasia
Rupture
Pathology
Inflammation

Keywords

  • Aging
  • Emphysema
  • Macrophages
  • Ozone
  • Surfactant protein D

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation. / Groves, Angela M.; Gow, Andrew J.; Massa, Christopher B.; Hall, Le Roy; Laskin, Jeffrey D.; Laskin, Debra L.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 305, No. 8, 15.10.2013.

Research output: Contribution to journalArticle

Groves, Angela M. ; Gow, Andrew J. ; Massa, Christopher B. ; Hall, Le Roy ; Laskin, Jeffrey D. ; Laskin, Debra L. / Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2013 ; Vol. 305, No. 8.
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