Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications

J. Löfblom, J. Feldwisch, V. Tolmachev, J. Carlsson, S. Ståhl, F. Y. Frejd

Research output: Contribution to journalReview articlepeer-review

396 Citations (Scopus)

Abstract

Affibody molecules are a class of engineered affinity proteins with proven potential for therapeutic, diagnostic and biotechnological applications. Affibody molecules are small (6.5. kDa) single domain proteins that can be isolated for high affinity and specificity to any given protein target. Fifteen years after its discovery, the Affibody technology is gaining use in many groups as a tool for creating molecular specificity wherever a small, engineering compatible tool is warranted. Here we summarize recent results using this technology, propose an Affibody nomenclature and give an overview of different HER2-specific Affibody molecules. Cumulative evidence suggests that the three helical scaffold domain used as basis for these molecules is highly suited to create a molecular affinity handle for vastly different applications.

Original languageEnglish
Pages (from-to)2670-2680
Number of pages11
JournalFEBS Letters
Volume584
Issue number12
DOIs
Publication statusPublished - Jun 2010
Externally publishedYes

Keywords

  • Affibody molecule
  • Alternative scaffold
  • Biotherapy
  • Combinatorial protein engineering
  • Molecular imaging
  • Phage display

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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