Creation of hydrophobic and hydrophilic surfaces with the spatially selective distribution of grafted organic moieties have great potential in the field of medicine and avionics. The one of more scalable approaches for spatially selective surface modification is based on colloid lithography, which allows large throughput for the subsequent surface grafting and realization of periodic chemical pattern. In this work, we demonstrated the spatially-selective surface grafting by ADT salt with hydrophobic -C8F17 or hydrophilic -COOH groups using following procedure: (i) deposition of PS colloid suspension on substrate; (ii) electrochemical modification of surface with diazonium salt; (iii) removing of PS mask; (iv) second electrochemical modification with diazonium salt. The surface modification through the mask was controlled by PS size, that allowed tuning the area, covered in the first stage of grafting. The modified surface morphology and chemistry was measured by IR spectroscopy and AFM microscopy. Proposed approach allows to effectively decorate surface with different chemical groups in an accurate and precise manner, with additional feature of their mutual “periodic” arrangement.