Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles

Ekaterina A. Chudinova, Maria A. Surmeneva, Alexander S. Timin, Timofey E. Karpov, Alexandra Wittmar, Mathias Ulbricht, Anna Ivanova, Kateryna Loza, Oleg Prymak, Andrey Koptyug, Matthias Epple, Roman A. Surmenev

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.

Original languageEnglish
Pages (from-to)130-139
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume176
DOIs
Publication statusPublished - 1 Apr 2019

Fingerprint

calcium phosphates
stem cells
Calcium phosphate
Stem cells
Mesenchymal Stromal Cells
Scaffolds
Nanoparticles
adhesion
Adhesion
nanoparticles
Polyethyleneimine
Cells
Electron beam melting
titanium alloy (TiAl6V4)
calcium phosphate
phosphatases
Surface Properties
Phosphatases
Hydrophilicity
Cell proliferation

Keywords

  • Additive manufacturing
  • Calcium phosphate
  • Cell adhesion
  • Electron beam melting
  • Electrophoretic deposition
  • Nanoparticles
  • Proliferation in vivo
  • Scaffold
  • Surface properties

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles. / Chudinova, Ekaterina A.; Surmeneva, Maria A.; Timin, Alexander S.; Karpov, Timofey E.; Wittmar, Alexandra; Ulbricht, Mathias; Ivanova, Anna; Loza, Kateryna; Prymak, Oleg; Koptyug, Andrey; Epple, Matthias; Surmenev, Roman A.

In: Colloids and Surfaces B: Biointerfaces, Vol. 176, 01.04.2019, p. 130-139.

Research output: Contribution to journalArticle

@article{9eb98d95625b4bc3b7a84f60d567084d,
title = "Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles",
abstract = "In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.",
keywords = "Additive manufacturing, Calcium phosphate, Cell adhesion, Electron beam melting, Electrophoretic deposition, Nanoparticles, Proliferation in vivo, Scaffold, Surface properties",
author = "Chudinova, {Ekaterina A.} and Surmeneva, {Maria A.} and Timin, {Alexander S.} and Karpov, {Timofey E.} and Alexandra Wittmar and Mathias Ulbricht and Anna Ivanova and Kateryna Loza and Oleg Prymak and Andrey Koptyug and Matthias Epple and Surmenev, {Roman A.}",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.colsurfb.2018.12.047",
language = "English",
volume = "176",
pages = "130--139",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles

AU - Chudinova, Ekaterina A.

AU - Surmeneva, Maria A.

AU - Timin, Alexander S.

AU - Karpov, Timofey E.

AU - Wittmar, Alexandra

AU - Ulbricht, Mathias

AU - Ivanova, Anna

AU - Loza, Kateryna

AU - Prymak, Oleg

AU - Koptyug, Andrey

AU - Epple, Matthias

AU - Surmenev, Roman A.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.

AB - In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.

KW - Additive manufacturing

KW - Calcium phosphate

KW - Cell adhesion

KW - Electron beam melting

KW - Electrophoretic deposition

KW - Nanoparticles

KW - Proliferation in vivo

KW - Scaffold

KW - Surface properties

UR - http://www.scopus.com/inward/record.url?scp=85059137646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059137646&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2018.12.047

DO - 10.1016/j.colsurfb.2018.12.047

M3 - Article

AN - SCOPUS:85059137646

VL - 176

SP - 130

EP - 139

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -