Abstract
In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.
| Original language | English |
|---|---|
| Pages (from-to) | 130-139 |
| Number of pages | 10 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 176 |
| DOIs | |
| Publication status | Published - 1 Apr 2019 |
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Keywords
- Additive manufacturing
- Calcium phosphate
- Cell adhesion
- Electron beam melting
- Electrophoretic deposition
- Nanoparticles
- Proliferation in vivo
- Scaffold
- Surface properties
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles. / Chudinova, Ekaterina A.; Surmeneva, Maria A.; Timin, Alexander S.; Karpov, Timofey E.; Wittmar, Alexandra; Ulbricht, Mathias; Ivanova, Anna; Loza, Kateryna; Prymak, Oleg; Koptyug, Andrey; Epple, Matthias; Surmenev, Roman A.
In: Colloids and Surfaces B: Biointerfaces, Vol. 176, 01.04.2019, p. 130-139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells on additively manufactured Ti6Al4V alloy scaffolds modified with calcium phosphate nanoparticles
AU - Chudinova, Ekaterina A.
AU - Surmeneva, Maria A.
AU - Timin, Alexander S.
AU - Karpov, Timofey E.
AU - Wittmar, Alexandra
AU - Ulbricht, Mathias
AU - Ivanova, Anna
AU - Loza, Kateryna
AU - Prymak, Oleg
AU - Koptyug, Andrey
AU - Epple, Matthias
AU - Surmenev, Roman A.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.
AB - In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.
KW - Additive manufacturing
KW - Calcium phosphate
KW - Cell adhesion
KW - Electron beam melting
KW - Electrophoretic deposition
KW - Nanoparticles
KW - Proliferation in vivo
KW - Scaffold
KW - Surface properties
UR - http://www.scopus.com/inward/record.url?scp=85059137646&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059137646&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2018.12.047
DO - 10.1016/j.colsurfb.2018.12.047
M3 - Article
VL - 176
SP - 130
EP - 139
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -