Abstract
Recombinant human bone morphogenetic protein 2 (rhBMP-2) has been widely employed for the induction of bone growth in animal models and in clinical trials. Since rhBMP-2 is commercially available only to a very limited extent, we have prepared our own recombinant material for the current testing and for future applications. As yet, no toxicological data on rhBMP-2 has been reported to the knowledge of the authors. Therefore, it was considered necessary to apply the in vifro-micronucleus assay to our preparation to monitor a possible genotoxic effect. A total of approximately 24500 nuclei from V79 Chinese hamster cells and more than 40000 nuclei from human amniotic fluid fibroblast-like (AFFL) cells were screened for the frequency of micronuclei following incubation with 3-300 nM rhBMP-2, which covers the concentration range from initial biological activity to the begin of a maximal response in MC3T3-E1 cells. It can be concluded from the results that our preparations of recombinant human BMP-2 prepared in E. coli do not cause DNA damage in the concentration range tested.
Original language | English |
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Pages (from-to) | 1101-1105 |
Number of pages | 5 |
Journal | Materialwissenschaft und Werkstofftechnik |
Volume | 34 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2003 |
Externally published | Yes |
Keywords
- BMP-2
- Cisplatin
- Micronucleus
- Mitosis
- Mutagenesis
- Toxicology
- V79 hamster
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering