Abstract
The aim of the investigation was to create multifunctional antitumor protein agent DARPin-mCherry-PE40 possessing both diagnostic and therapeutic properties. Materials and Methods. The experiments were carried out on human cell lines of breast adenocarcinoma SK-BR-3. Chinese hamster ovarian cell line CHO-K1 was used as a control. Genetic engineering methods, metal chelate affinity chromatography, confocal microscopy and spectrophotometric MTT test were used to perform the experiments. Results. A multifunctional targeted anticancer protein agent DARPin-mCherry-PE40 consisting of targeted HER2-specific nonimmunoglobulin module DARPin, an imaging module mCherry and cytotoxic module based on a fragment of Pseudomonas exotoxin A (PE40) has been created. Using HER2-positive cells it was shown in vitro that all three domains of the recombinant protein retained their functional qualities — high affinity for HER2 antigen, ability to fluorescence, and cytotoxicity. Conclusion. The ability of the targeted antitumor agent DARPin-mCherry-PE40 selectively stains HER2-positive cells and highly selective cytotoxicity against these cells make this targeted recombinant protein DARPin-mCherry-PE40 a perspective theranostic agent for the diagnosis and treatment of HER2-positive human tumors.
| Original language | English |
|---|---|
| Pages (from-to) | 7-12 |
| Number of pages | 6 |
| Journal | Sovremennye Tehnologii v Medicine |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2016 |
Fingerprint
Keywords
- DARPin
- HER2 receptor
- MCherry
- PE40
- Pseudomonas aeruginosa exotoxin A fragment
- Theranostics
ASJC Scopus subject areas
- Medicine(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
A targeted toxin based on a binding module of non-immunoglobulin scaffold as a new agent for theranostics. / Proshkina, Galina M.; Stremovskiy, O. A.; Sibrikova, D. V.; Ryabova, A. V.; Deyev, S. M.
In: Sovremennye Tehnologii v Medicine, Vol. 8, No. 4, 2016, p. 7-12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A targeted toxin based on a binding module of non-immunoglobulin scaffold as a new agent for theranostics
AU - Proshkina, Galina M.
AU - Stremovskiy, O. A.
AU - Sibrikova, D. V.
AU - Ryabova, A. V.
AU - Deyev, S. M.
PY - 2016
Y1 - 2016
N2 - The aim of the investigation was to create multifunctional antitumor protein agent DARPin-mCherry-PE40 possessing both diagnostic and therapeutic properties. Materials and Methods. The experiments were carried out on human cell lines of breast adenocarcinoma SK-BR-3. Chinese hamster ovarian cell line CHO-K1 was used as a control. Genetic engineering methods, metal chelate affinity chromatography, confocal microscopy and spectrophotometric MTT test were used to perform the experiments. Results. A multifunctional targeted anticancer protein agent DARPin-mCherry-PE40 consisting of targeted HER2-specific nonimmunoglobulin module DARPin, an imaging module mCherry and cytotoxic module based on a fragment of Pseudomonas exotoxin A (PE40) has been created. Using HER2-positive cells it was shown in vitro that all three domains of the recombinant protein retained their functional qualities — high affinity for HER2 antigen, ability to fluorescence, and cytotoxicity. Conclusion. The ability of the targeted antitumor agent DARPin-mCherry-PE40 selectively stains HER2-positive cells and highly selective cytotoxicity against these cells make this targeted recombinant protein DARPin-mCherry-PE40 a perspective theranostic agent for the diagnosis and treatment of HER2-positive human tumors.
AB - The aim of the investigation was to create multifunctional antitumor protein agent DARPin-mCherry-PE40 possessing both diagnostic and therapeutic properties. Materials and Methods. The experiments were carried out on human cell lines of breast adenocarcinoma SK-BR-3. Chinese hamster ovarian cell line CHO-K1 was used as a control. Genetic engineering methods, metal chelate affinity chromatography, confocal microscopy and spectrophotometric MTT test were used to perform the experiments. Results. A multifunctional targeted anticancer protein agent DARPin-mCherry-PE40 consisting of targeted HER2-specific nonimmunoglobulin module DARPin, an imaging module mCherry and cytotoxic module based on a fragment of Pseudomonas exotoxin A (PE40) has been created. Using HER2-positive cells it was shown in vitro that all three domains of the recombinant protein retained their functional qualities — high affinity for HER2 antigen, ability to fluorescence, and cytotoxicity. Conclusion. The ability of the targeted antitumor agent DARPin-mCherry-PE40 selectively stains HER2-positive cells and highly selective cytotoxicity against these cells make this targeted recombinant protein DARPin-mCherry-PE40 a perspective theranostic agent for the diagnosis and treatment of HER2-positive human tumors.
KW - DARPin
KW - HER2 receptor
KW - MCherry
KW - PE40
KW - Pseudomonas aeruginosa exotoxin A fragment
KW - Theranostics
UR - http://www.scopus.com/inward/record.url?scp=85007489713&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007489713&partnerID=8YFLogxK
U2 - 10.17691/stm2016.8.4.01
DO - 10.17691/stm2016.8.4.01
M3 - Article
VL - 8
SP - 7
EP - 12
JO - Sovremennye Tehnologii v Medicine
JF - Sovremennye Tehnologii v Medicine
SN - 2076-4243
IS - 4
ER -