A study of the mechanism of no-dependent smooth muscle relaxation in rat aorta under the action of nitrovasodilators

I. V. Kovalev, A. G. Popov, A. A. Panov, Yu L. Borodin, Yana Jonovna Anfinogenova, L. V. Kapilevich, M. B. Baskakov, M. A. Medvedev

Research output: Contribution to journalArticle

Abstract

The membrane potential and smooth muscle tension in rat aorta were studied by the method of sucrose gap junction. It was found that sodium nitroprusside and nitroglycerin produced a dose-dependent membrane repolarization and smooth muscle cell relaxation in rat aorta preliminarily contracted and depolarized by hyperpotassium (40 mM) or phenylephrine solutions. The relaxation effect of sodium nitroprusside was more pronounced on the phenylephrine background. The effect of nitroglycerin showed a different kinetics in time and led to the tolerance development. The effects of both nitro compounds were inhibited by pretreatment with Methylene Blue or potassium channel blockers. It is suggested that nitrovasodilators are involved in the NO-dependent processes in smoothmuscle cells of aorta through cGMP-mediated activation of the potassium conductivity and by changing the efficiency of operation of the protein kinase C branch of the Ca2+ signal system.

Original languageEnglish
Pages (from-to)36
Number of pages1
JournalEksperimental'naya i Klinicheskaya Farmakologiya
Volume64
Issue number3
Publication statusPublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

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