A study of HPLC separation and spectrophotometric and voltammetric detection of 4′-substituted derivatives of 3-carboxy-4-hydroxy-6-acetylaminoazobenzene

Jiří Barek, Josef Cvačka, Jiří Zima

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1 Citation (Scopus)

Abstract

Optimised conditions have been found for the separation of 3-carboxy-4-hydroxy-6-acetylaminoazobenzene derivatives substituted in the position 4′ (4′-R-CHAAB, where R = -H, -CH3, -OCH3, -Cl, -COCH3, -NO2 and -NHCOCH3) using reversed phase HPLC with a C18 chemically bonded stationary phase. Suitable mobile phases are mixtures of 0.01 mol/L NaH2PO4 at pH 4 with methanol (1 + 1), 0.01 mol/L NaH2PO4 at pH 2 with acetonitrile (1 + 1) or 1% aqueous acetic acid with methanol (4 + 6). UV photometry is the most universal detection technique and yields limits of detection around 10-6 mol/L. Direct anodic voltammetry on a glassy carbon fibre array detector yields lower limits of detection for -COCH3 derivatives and higher limits of detection for -NO2 and -NHCOCH3 derivatives. When the analytes are chemically reduced using zinc powder in acetic acid, the voltammetric detection has limits of detection one order of magnitude lower than those obtained UV photometrically.

Original languageEnglish
Pages (from-to)493-499
Number of pages7
JournalFresenius' Journal of Analytical Chemistry
Volume358
Issue number4
DOIs
Publication statusPublished - 1 Jan 1997
Externally publishedYes

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Limit of Detection
High Pressure Liquid Chromatography
Derivatives
Acetic Acid
Methanol
Photometry
Glassy carbon
Voltammetry
Powders
Zinc
Detectors
carbon fiber
acetonitrile

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "A study of HPLC separation and spectrophotometric and voltammetric detection of 4′-substituted derivatives of 3-carboxy-4-hydroxy-6-acetylaminoazobenzene",
abstract = "Optimised conditions have been found for the separation of 3-carboxy-4-hydroxy-6-acetylaminoazobenzene derivatives substituted in the position 4′ (4′-R-CHAAB, where R = -H, -CH3, -OCH3, -Cl, -COCH3, -NO2 and -NHCOCH3) using reversed phase HPLC with a C18 chemically bonded stationary phase. Suitable mobile phases are mixtures of 0.01 mol/L NaH2PO4 at pH 4 with methanol (1 + 1), 0.01 mol/L NaH2PO4 at pH 2 with acetonitrile (1 + 1) or 1{\%} aqueous acetic acid with methanol (4 + 6). UV photometry is the most universal detection technique and yields limits of detection around 10-6 mol/L. Direct anodic voltammetry on a glassy carbon fibre array detector yields lower limits of detection for -COCH3 derivatives and higher limits of detection for -NO2 and -NHCOCH3 derivatives. When the analytes are chemically reduced using zinc powder in acetic acid, the voltammetric detection has limits of detection one order of magnitude lower than those obtained UV photometrically.",
author = "Jiř{\'i} Barek and Josef Cvačka and Jiř{\'i} Zima",
year = "1997",
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T1 - A study of HPLC separation and spectrophotometric and voltammetric detection of 4′-substituted derivatives of 3-carboxy-4-hydroxy-6-acetylaminoazobenzene

AU - Barek, Jiří

AU - Cvačka, Josef

AU - Zima, Jiří

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Optimised conditions have been found for the separation of 3-carboxy-4-hydroxy-6-acetylaminoazobenzene derivatives substituted in the position 4′ (4′-R-CHAAB, where R = -H, -CH3, -OCH3, -Cl, -COCH3, -NO2 and -NHCOCH3) using reversed phase HPLC with a C18 chemically bonded stationary phase. Suitable mobile phases are mixtures of 0.01 mol/L NaH2PO4 at pH 4 with methanol (1 + 1), 0.01 mol/L NaH2PO4 at pH 2 with acetonitrile (1 + 1) or 1% aqueous acetic acid with methanol (4 + 6). UV photometry is the most universal detection technique and yields limits of detection around 10-6 mol/L. Direct anodic voltammetry on a glassy carbon fibre array detector yields lower limits of detection for -COCH3 derivatives and higher limits of detection for -NO2 and -NHCOCH3 derivatives. When the analytes are chemically reduced using zinc powder in acetic acid, the voltammetric detection has limits of detection one order of magnitude lower than those obtained UV photometrically.

AB - Optimised conditions have been found for the separation of 3-carboxy-4-hydroxy-6-acetylaminoazobenzene derivatives substituted in the position 4′ (4′-R-CHAAB, where R = -H, -CH3, -OCH3, -Cl, -COCH3, -NO2 and -NHCOCH3) using reversed phase HPLC with a C18 chemically bonded stationary phase. Suitable mobile phases are mixtures of 0.01 mol/L NaH2PO4 at pH 4 with methanol (1 + 1), 0.01 mol/L NaH2PO4 at pH 2 with acetonitrile (1 + 1) or 1% aqueous acetic acid with methanol (4 + 6). UV photometry is the most universal detection technique and yields limits of detection around 10-6 mol/L. Direct anodic voltammetry on a glassy carbon fibre array detector yields lower limits of detection for -COCH3 derivatives and higher limits of detection for -NO2 and -NHCOCH3 derivatives. When the analytes are chemically reduced using zinc powder in acetic acid, the voltammetric detection has limits of detection one order of magnitude lower than those obtained UV photometrically.

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