A novel far-red fluorescent xenograft model of ovarian carcinoma for preclinical evaluation of HER2-targeted immunotoxins

Tatiana Zdobnova, Evgeniya Sokolova, Oleg Stremovskiy, Dmitry Karpenko, William Telford, Ilya Turchin, Irina Balalaeva, Sergey Deyev

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have created a novel fluorescent model of a human ovarian carcinoma xenograft overexpressing receptor HER2, a promising molecular target of solid tumors. The model is based on a newly generated SKOV-kat cell line stably expressing far-red fluorescent protein Katushka. Katushka is most suitable for the in vivo imaging due to an optimal combination of high brightness and emission in the "window of tissue transparency". The relevance of the fluorescent model for the in vivo monitoring of tumor growth and response to treatment was demonstrated using a newly created HER2-targeted recombinant immunotoxin based on the 4D5scFv antibody and a fragment of the Pseudomonas exotoxin A.

Original languageEnglish
Pages (from-to)30919-30928
Number of pages10
JournalOncotarget
Volume6
Issue number31
DOIs
Publication statusPublished - 2015
Externally publishedYes

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Keywords

  • Far-red fluorescent protein
  • HER2
  • Immunotoxin
  • Ovarian carcinoma
  • Xenograft tumor model

ASJC Scopus subject areas

  • Oncology

Cite this

Zdobnova, T., Sokolova, E., Stremovskiy, O., Karpenko, D., Telford, W., Turchin, I., Balalaeva, I., & Deyev, S. (2015). A novel far-red fluorescent xenograft model of ovarian carcinoma for preclinical evaluation of HER2-targeted immunotoxins. Oncotarget, 6(31), 30919-30928. https://doi.org/10.18632/oncotarget.5130