A nitric oxide processing defect of red blood cells created by hypoxia: Deficiency of S-nitrosohemoglobin in pulmonary hypertension

Timothy J. McMahon, Gregory S. Ahearn, Martin P. Moya, Andrew J. Gow, Yuh Chin T Huang, Benjamin P. Luchsinger, Raphael Nudelman, Yun Yan, Abigail D. Krichman, Thomas M. Bashore, Robert M. Califf, David J. Singel, Claude A. Piantadosi, Victor F. Tapson, Jonathan S. Stamler

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The mechanism by which hypoxia [low partial pressure of O2 (pO2)] elicits signaling to regulate pulmonary arterial pressure is incompletely understood. We considered the possibility that, in addition to its effects on smooth muscle, hypoxia may influence pulmonary vascular tone through an effect on RBCs. We report that exposure of native RBCs to sustained hypoxia is accompanied by a buildup of heme iron-nitrosyl (FeNO) species that are deficient in pO2-governed intramolecular transfer of NO to cysteine thiol, yielding a deficiency in the vasodilator S-nitrosohemoglobin (SNO-Hb). S-nitrosothiol (SNO)-deficient RBCs produce impaired vasodilator responses in vitro and exaggerated pulmonary vasoconstrictor responses in vivo and are defective in oxygenating the blood. RBCs from hypoxemic patients with elevated pulmonary arterial pressure (PAP) exhibit a similar FeNO/SNO imbalance and are thus deficient in pO2-coupled vasoregulation. Chemical restoration of SNO-Hb levels in both animals and patients restores the vasodilator activity of RBCs, and this activity is associated with improved oxygenation and lower PAPs.

Original languageEnglish
Pages (from-to)14801-14806
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number41
DOIs
Publication statusPublished - 11 Oct 2005
Externally publishedYes

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S-Nitrosothiols
Cell Hypoxia
Pulmonary Hypertension
Nitric Oxide
Erythrocytes
Vasodilator Agents
Lung
Arterial Pressure
Pressure
Partial Pressure
Vasoconstrictor Agents
Heme
Sulfhydryl Compounds
Cysteine
Smooth Muscle
Blood Vessels
S-nitrosohemoglobin
Hypoxia

Keywords

  • Hemoglobin
  • Red blood cell vasodilation
  • S-nitrosylation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A nitric oxide processing defect of red blood cells created by hypoxia : Deficiency of S-nitrosohemoglobin in pulmonary hypertension. / McMahon, Timothy J.; Ahearn, Gregory S.; Moya, Martin P.; Gow, Andrew J.; Huang, Yuh Chin T; Luchsinger, Benjamin P.; Nudelman, Raphael; Yan, Yun; Krichman, Abigail D.; Bashore, Thomas M.; Califf, Robert M.; Singel, David J.; Piantadosi, Claude A.; Tapson, Victor F.; Stamler, Jonathan S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 41, 11.10.2005, p. 14801-14806.

Research output: Contribution to journalArticle

McMahon, TJ, Ahearn, GS, Moya, MP, Gow, AJ, Huang, YCT, Luchsinger, BP, Nudelman, R, Yan, Y, Krichman, AD, Bashore, TM, Califf, RM, Singel, DJ, Piantadosi, CA, Tapson, VF & Stamler, JS 2005, 'A nitric oxide processing defect of red blood cells created by hypoxia: Deficiency of S-nitrosohemoglobin in pulmonary hypertension', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 41, pp. 14801-14806. https://doi.org/10.1073/pnas.0506957102
McMahon, Timothy J. ; Ahearn, Gregory S. ; Moya, Martin P. ; Gow, Andrew J. ; Huang, Yuh Chin T ; Luchsinger, Benjamin P. ; Nudelman, Raphael ; Yan, Yun ; Krichman, Abigail D. ; Bashore, Thomas M. ; Califf, Robert M. ; Singel, David J. ; Piantadosi, Claude A. ; Tapson, Victor F. ; Stamler, Jonathan S. / A nitric oxide processing defect of red blood cells created by hypoxia : Deficiency of S-nitrosohemoglobin in pulmonary hypertension. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 41. pp. 14801-14806.
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