A new restriction endonuclease from Citrobacter freundii

A. A. Janulaitis, P. S. Stakenas, E. N. Lebedenko, Yu A. Berlin

Research output: Contribution to journal › Article › peer-review

Abstract

CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacter freundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5′ -protruding tetranucleotide ends GGCC.

Original language	English
Pages (from-to)	6521-6530
Number of pages	10
Journal	Nucleic Acids Research
Volume	10
Issue number	20
DOIs	https://doi.org/10.1093/nar/10.20.6521
Publication status	Published - 25 Oct 1982
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "A new restriction endonuclease from Citrobacter freundii",

abstract = "CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacter freundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5′ -protruding tetranucleotide ends GGCC.",

author = "Janulaitis, {A. A.} and Stakenas, {P. S.} and Lebedenko, {E. N.} and Berlin, {Yu A.}",

year = "1982",

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AU - Lebedenko, E. N.

AU - Berlin, Yu A.

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N2 - CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacter freundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5′ -protruding tetranucleotide ends GGCC.

AB - CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacter freundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5′ -protruding tetranucleotide ends GGCC.

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