A new restriction endonuclease from Citrobacter freundii

A. A. Janulaitis, P. S. Stakenas, E. N. Lebedenko, Yu A. Berlin

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacter freundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5′ -protruding tetranucleotide ends GGCC.

Original languageEnglish
Pages (from-to)6521-6530
Number of pages10
JournalNucleic Acids Research
Volume10
Issue number20
DOIs
Publication statusPublished - 25 Oct 1982
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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