Abstract
Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.
| Original language | English |
|---|---|
| Pages (from-to) | 116-122 |
| Number of pages | 7 |
| Journal | Biochimie |
| Volume | 118 |
| DOIs | |
| Publication status | Published - 9 Nov 2015 |
| Externally published | Yes |
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Keywords
- Blue light illumination
- DARPin
- HER2/neu
- miniSOG
ASJC Scopus subject areas
- Biochemistry
Cite this
A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG. / Proshkina, G. M.; Shilova, O. N.; Ryabova, A. V.; Stremovskiy, O. A.; Deyev, S. M.
In: Biochimie, Vol. 118, 09.11.2015, p. 116-122.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG
AU - Proshkina, G. M.
AU - Shilova, O. N.
AU - Ryabova, A. V.
AU - Stremovskiy, O. A.
AU - Deyev, S. M.
PY - 2015/11/9
Y1 - 2015/11/9
N2 - Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.
AB - Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.
KW - Blue light illumination
KW - DARPin
KW - HER2/neu
KW - miniSOG
UR - http://www.scopus.com/inward/record.url?scp=84941089642&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941089642&partnerID=8YFLogxK
U2 - 10.1016/j.biochi.2015.08.013
DO - 10.1016/j.biochi.2015.08.013
M3 - Article
VL - 118
SP - 116
EP - 122
JO - Biochimie
JF - Biochimie
SN - 0300-9084
ER -