Abstract
Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.
Original language | English |
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Pages (from-to) | 116-122 |
Number of pages | 7 |
Journal | Biochimie |
Volume | 118 |
DOIs | |
Publication status | Published - 9 Nov 2015 |
Externally published | Yes |
Keywords
- Blue light illumination
- DARPin
- HER2/neu
- miniSOG
ASJC Scopus subject areas
- Biochemistry