A mass spectrometry plate reader: Monitoring enzyme activity and inhibition with a desorption/ionization on silicon (DIOS) platform

Abstract

A surface-based laser desorption/ionization mass spectrometry assay that makes use of Desorption/Ionization on Silicon Mass Spectrometry (DIOS-MS) has been developed to monitor enzyme activity and enzyme inhibition. DIOS-MS has been used to characterize inhibitors from a library and then to monitor their activity against selected enzyme targets, including proteases, glycotransferase, and acetylcholinesterase. An automated DIOS-MS system was also used as a high-throughput screen for the activity of novel enzymes and enzyme inhibitors. On two different commercially available instruments, a sampling rate of up to 38 inhibitors per minute was accomplished, with thousands of inhibitors being monitored. The ease of applying mass spectrometry toward developing enzyme assays and the speed of surface-based assays such as DIOS for monitoring inhibitor effectiveness and enzyme activity makes it attractive for a broad range of screening applications.

Original language	English
Pages (from-to)	921-927
Number of pages	7
Journal	ChemBioChem
Volume	5
Issue number	7
DOIs	https://doi.org/10.1002/cbic.200400008
Publication status	Published - 5 Jul 2004
Externally published	Yes

Keywords

DIOS
Enzymes
High-throughput screening
Inhibitors
Plate reader

ASJC Scopus subject areas

Drug Discovery
Organic Chemistry
Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1002/cbic.200400008

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Shen, Z., Go, E. P., Gamez, A., Apon, J. V., Fokin, V., Greig, M., Ventura, M., Crowell, J. E., Blixt, O., Paulson, J. C., Stevens, R. C., Finn, M. G., & Siuzdak, G. (2004). A mass spectrometry plate reader: Monitoring enzyme activity and inhibition with a desorption/ionization on silicon (DIOS) platform. ChemBioChem, 5(7), 921-927. https://doi.org/10.1002/cbic.200400008

A mass spectrometry plate reader : Monitoring enzyme activity and inhibition with a desorption/ionization on silicon (DIOS) platform. / Shen, Zhouxin; Go, Eden P.; Gamez, Alejandra; Apon, Junefredo V.; Fokin, Valery; Greig, Mike; Ventura, Manuel; Crowell, John E.; Blixt, Ola; Paulson, James C.; Stevens, Raymond C.; Finn, M. G.; Siuzdak, Gary.

In: ChemBioChem, Vol. 5, No. 7, 05.07.2004, p. 921-927.

Research output: Contribution to journal › Article › peer-review

Shen, Zhouxin ; Go, Eden P. ; Gamez, Alejandra ; Apon, Junefredo V. ; Fokin, Valery ; Greig, Mike ; Ventura, Manuel ; Crowell, John E. ; Blixt, Ola ; Paulson, James C. ; Stevens, Raymond C. ; Finn, M. G. ; Siuzdak, Gary. / A mass spectrometry plate reader : Monitoring enzyme activity and inhibition with a desorption/ionization on silicon (DIOS) platform. In: ChemBioChem. 2004 ; Vol. 5, No. 7. pp. 921-927.

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abstract = "A surface-based laser desorption/ionization mass spectrometry assay that makes use of Desorption/Ionization on Silicon Mass Spectrometry (DIOS-MS) has been developed to monitor enzyme activity and enzyme inhibition. DIOS-MS has been used to characterize inhibitors from a library and then to monitor their activity against selected enzyme targets, including proteases, glycotransferase, and acetylcholinesterase. An automated DIOS-MS system was also used as a high-throughput screen for the activity of novel enzymes and enzyme inhibitors. On two different commercially available instruments, a sampling rate of up to 38 inhibitors per minute was accomplished, with thousands of inhibitors being monitored. The ease of applying mass spectrometry toward developing enzyme assays and the speed of surface-based assays such as DIOS for monitoring inhibitor effectiveness and enzyme activity makes it attractive for a broad range of screening applications.",

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author = "Zhouxin Shen and Go, {Eden P.} and Alejandra Gamez and Apon, {Junefredo V.} and Valery Fokin and Mike Greig and Manuel Ventura and Crowell, {John E.} and Ola Blixt and Paulson, {James C.} and Stevens, {Raymond C.} and Finn, {M. G.} and Gary Siuzdak",

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AU - Fokin, Valery

AU - Greig, Mike

AU - Ventura, Manuel

AU - Crowell, John E.

AU - Blixt, Ola

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AU - Stevens, Raymond C.

AU - Finn, M. G.

AU - Siuzdak, Gary

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AB - A surface-based laser desorption/ionization mass spectrometry assay that makes use of Desorption/Ionization on Silicon Mass Spectrometry (DIOS-MS) has been developed to monitor enzyme activity and enzyme inhibition. DIOS-MS has been used to characterize inhibitors from a library and then to monitor their activity against selected enzyme targets, including proteases, glycotransferase, and acetylcholinesterase. An automated DIOS-MS system was also used as a high-throughput screen for the activity of novel enzymes and enzyme inhibitors. On two different commercially available instruments, a sampling rate of up to 38 inhibitors per minute was accomplished, with thousands of inhibitors being monitored. The ease of applying mass spectrometry toward developing enzyme assays and the speed of surface-based assays such as DIOS for monitoring inhibitor effectiveness and enzyme activity makes it attractive for a broad range of screening applications.

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KW - High-throughput screening

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KW - Plate reader

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