A large-scale expression in Escherichia coli of neurotoxin II from Naja oxiana fused with thioredoxin

E. N. Lyukmanova, A. A. Shulga, D. A. Arsenieva, K. A. Pluzhnikov, D. A. Dolgikh, A. S. Arseniev, M. P. Kirpichnikov

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Neurotoxin II from the venom of cobra Naja oxiana is a short type α-neurotoxin, which competitively inhibits nicotinic acetylcholine receptor. The toxin gene was expressed as a construct fused with the thioredoxin gene and the linker encoding the enteropeptidase recognition site and a Met residue between the genes. The fusion protein was mainly cleaved by cyanogen bromide, since enteropeptidase was less effective. The yield of neurotoxin II was 6 mg/l of the bacterial culture. The resulting recombinant protein was identified with native neurotoxin II by its N-terminal analysis, mass spectrometry, and NMR spectroscopy.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalRussian Journal of Bioorganic Chemistry
Volume30
Issue number1
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

Keywords

  • α-neurotoxins
  • Expression in Escherichia coli
  • Nicotinic acetylcholine receptor
  • Thioredoxin

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'A large-scale expression in Escherichia coli of neurotoxin II from Naja oxiana fused with thioredoxin'. Together they form a unique fingerprint.

Cite this