Neurotoxin II from the venom of cobra Naja oxiana is a short type α-neurotoxin, which competitively inhibits nicotinic acetylcholine receptor. The toxin gene was expressed as a construct fused with the thioredoxin gene and the linker encoding the enteropeptidase recognition site and a Met residue between the genes. The fusion protein was mainly cleaved by cyanogen bromide, since enteropeptidase was less effective. The yield of neurotoxin II was 6 mg/l of the bacterial culture. The resulting recombinant protein was identified with native neurotoxin II by its N-terminal analysis, mass spectrometry, and NMR spectroscopy.
|Number of pages||10|
|Journal||Russian Journal of Bioorganic Chemistry|
|Publication status||Published - Jan 2004|
- Expression in Escherichia coli
- Nicotinic acetylcholine receptor
ASJC Scopus subject areas
- Organic Chemistry