TY - JOUR
T1 - A high-level prokaryotic expression system
T2 - synthesis of human interleukin 1α and its receptor antagonist
AU - Birikh, Klara R.
AU - Lebedenko, Ekaterina N.
AU - Boni, Irina V.
AU - Berlin, Yuri A.
N1 - Funding Information:
We are gratefutl o O.V. Plutalova nd A.L. Kayushin for the oligo synthesisto, V.I. Makhnof or 30Sr ibosomal subunitsa ndto A.S. Simbirtsefvo r polyclonaal ntibodies to ILl~t. This work was supportedb y the Russian Foundationfo r FundamentaRle searc(hg rantN o. 94-03-08828a) ndby the StateP rogram" AdvancedM ethodsin Bioengineering".
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1995/10/27
Y1 - 1995/10/27
N2 - Synthetic intronless genes, coding for human interleukin 1α (IL1α) and interleukin 1 receptor antagonist (ILlra), have been expressed efficiently in a specially designed prokaryotic vector, pGMCE (a pGEM1 derivative), where the target gene forms the second part of a two-cistron system. The first part of the system is a translation enhancer-containing mini-cistron, whose termination codon overlaps the start codon of the target gene. In the case of the IL1α gene, the high expression level is largely due to the direct efficient translation initiation at the second cistron, whereas with the IL1ra gene in the same system, the proximal translation initiation region (TIR) provides a high level of coupled expression of the target gene. Thus, pGMCE is a potentially versatile vector for direct prokaryotic expression.
AB - Synthetic intronless genes, coding for human interleukin 1α (IL1α) and interleukin 1 receptor antagonist (ILlra), have been expressed efficiently in a specially designed prokaryotic vector, pGMCE (a pGEM1 derivative), where the target gene forms the second part of a two-cistron system. The first part of the system is a translation enhancer-containing mini-cistron, whose termination codon overlaps the start codon of the target gene. In the case of the IL1α gene, the high expression level is largely due to the direct efficient translation initiation at the second cistron, whereas with the IL1ra gene in the same system, the proximal translation initiation region (TIR) provides a high level of coupled expression of the target gene. Thus, pGMCE is a potentially versatile vector for direct prokaryotic expression.
KW - mini-cistron
KW - Recombinant DNA
KW - toeprinting
KW - translation initiation
KW - two-cistron expression system
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U2 - 10.1016/0378-1119(95)00488-R
DO - 10.1016/0378-1119(95)00488-R
M3 - Article
C2 - 7590354
AN - SCOPUS:0028858066
VL - 164
SP - 341
EP - 345
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -