A comparison study between electrospun polycaprolactone and piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffolds for bone tissue engineering

Svetlana N. Gorodzha, Albert R. Muslimov, Dina S. Syromotina, Alexander S. Timin, Nikolai Y. Tcvetkov, Kirill V. Lepik, Aleksandra V. Petrova, Maria A. Surmeneva, Dmitry A. Gorin, Gleb B. Sukhorukov, Roman A. Surmenev

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, bone scaffolds composed of polycaprolactone (PCL), piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and silicate containing hydroxyapatite (PHBV-SiHA) were successfully fabricated by a conventional electrospinning process. The morphological, chemical, wetting and biological properties of the scaffolds were examined. All fabricated scaffolds are composed of randomly oriented fibres with diameters from 800 nm to 12 μm. Fibre size increased with the addition of SiHA to PHBV scaffolds. Moreover, fibre surface roughness in the case of hybrid scaffolds was also increased. XRD, FTIR and Raman spectroscopy were used to analyse the chemical composition of the scaffolds, and contact angle measurements were performed to reveal the wetting behaviour of the synthesized materials. To determine the influence of the piezoelectric nature of PHBV in combination with SiHA nanoparticles on cell attachment and proliferation, PCL (non-piezoelectric), pure PHBV, and PHBV-SiHA scaffolds were seeded with human mesenchymal stem cells (hMSCs). In vitro study on hMSC adhesion, viability, spreading and osteogenic differentiation showed that the PHBV-SiHA scaffolds had the largest adhesion and differentiation abilities compared with other scaffolds. Moreover, the piezoelectric PHBV scaffolds have demonstrated better calcium deposition potential compared with non-piezoelectric PCL. The results of the study revealed pronounced advantages of hybrid PHBV-SiHA scaffolds to be used in bone tissue engineering.

Original languageEnglish
Pages (from-to)48-59
Number of pages12
JournalColloids and Surfaces B: Biointerfaces
Volume160
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Polycaprolactone
tissue engineering
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
bones
Bone
stem cells
Mesenchymal Stromal Cells
Bone and Bones
wetting
fibers
adhesion
Silicates
Aptitude
Raman Spectrum Analysis
Fourier Transform Infrared Spectroscopy
Durapatite
viability

Keywords

  • Cell adhesion
  • Electrospinning
  • Mineralization
  • Nanoparticles
  • Polymer scaffolds

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

A comparison study between electrospun polycaprolactone and piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffolds for bone tissue engineering. / Gorodzha, Svetlana N.; Muslimov, Albert R.; Syromotina, Dina S.; Timin, Alexander S.; Tcvetkov, Nikolai Y.; Lepik, Kirill V.; Petrova, Aleksandra V.; Surmeneva, Maria A.; Gorin, Dmitry A.; Sukhorukov, Gleb B.; Surmenev, Roman A.

In: Colloids and Surfaces B: Biointerfaces, Vol. 160, 01.12.2017, p. 48-59.

Research output: Contribution to journalArticle

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T1 - A comparison study between electrospun polycaprolactone and piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffolds for bone tissue engineering

AU - Gorodzha, Svetlana N.

AU - Muslimov, Albert R.

AU - Syromotina, Dina S.

AU - Timin, Alexander S.

AU - Tcvetkov, Nikolai Y.

AU - Lepik, Kirill V.

AU - Petrova, Aleksandra V.

AU - Surmeneva, Maria A.

AU - Gorin, Dmitry A.

AU - Sukhorukov, Gleb B.

AU - Surmenev, Roman A.

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N2 - In this study, bone scaffolds composed of polycaprolactone (PCL), piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and silicate containing hydroxyapatite (PHBV-SiHA) were successfully fabricated by a conventional electrospinning process. The morphological, chemical, wetting and biological properties of the scaffolds were examined. All fabricated scaffolds are composed of randomly oriented fibres with diameters from 800 nm to 12 μm. Fibre size increased with the addition of SiHA to PHBV scaffolds. Moreover, fibre surface roughness in the case of hybrid scaffolds was also increased. XRD, FTIR and Raman spectroscopy were used to analyse the chemical composition of the scaffolds, and contact angle measurements were performed to reveal the wetting behaviour of the synthesized materials. To determine the influence of the piezoelectric nature of PHBV in combination with SiHA nanoparticles on cell attachment and proliferation, PCL (non-piezoelectric), pure PHBV, and PHBV-SiHA scaffolds were seeded with human mesenchymal stem cells (hMSCs). In vitro study on hMSC adhesion, viability, spreading and osteogenic differentiation showed that the PHBV-SiHA scaffolds had the largest adhesion and differentiation abilities compared with other scaffolds. Moreover, the piezoelectric PHBV scaffolds have demonstrated better calcium deposition potential compared with non-piezoelectric PCL. The results of the study revealed pronounced advantages of hybrid PHBV-SiHA scaffolds to be used in bone tissue engineering.

AB - In this study, bone scaffolds composed of polycaprolactone (PCL), piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and silicate containing hydroxyapatite (PHBV-SiHA) were successfully fabricated by a conventional electrospinning process. The morphological, chemical, wetting and biological properties of the scaffolds were examined. All fabricated scaffolds are composed of randomly oriented fibres with diameters from 800 nm to 12 μm. Fibre size increased with the addition of SiHA to PHBV scaffolds. Moreover, fibre surface roughness in the case of hybrid scaffolds was also increased. XRD, FTIR and Raman spectroscopy were used to analyse the chemical composition of the scaffolds, and contact angle measurements were performed to reveal the wetting behaviour of the synthesized materials. To determine the influence of the piezoelectric nature of PHBV in combination with SiHA nanoparticles on cell attachment and proliferation, PCL (non-piezoelectric), pure PHBV, and PHBV-SiHA scaffolds were seeded with human mesenchymal stem cells (hMSCs). In vitro study on hMSC adhesion, viability, spreading and osteogenic differentiation showed that the PHBV-SiHA scaffolds had the largest adhesion and differentiation abilities compared with other scaffolds. Moreover, the piezoelectric PHBV scaffolds have demonstrated better calcium deposition potential compared with non-piezoelectric PCL. The results of the study revealed pronounced advantages of hybrid PHBV-SiHA scaffolds to be used in bone tissue engineering.

KW - Cell adhesion

KW - Electrospinning

KW - Mineralization

KW - Nanoparticles

KW - Polymer scaffolds

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