3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: High-resolution tomography and in vitro study

Svetlana Shkarina, Roman Shkarin, Venera Weinhardt, Elizaveta Melnik, Gabriele Vacun, Petra Kluger, Kateryna Loza, Matthias Epple, Sergei I. Ivlev, Tilo Baumbach, Maria A. Surmeneva, Roman A. Surmenev

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Abstract

To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 μm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue. Chemical and mechanical properties of the developed 3D scaffolds were investigated with XRD, FTIR, EDX and tensile testing. Furthermore, the internal structure and surface morphology of the scaffolds were analyzed using synchrotron X-ray μCT and SEM. Upon culturing human mesenchymal stem cells (hMSC) on PCL-SiHA scaffolds, we found that both SiHA inclusion and microfiber orientation affected cell adhesion. The best hMSCs viability was revealed at 10 day for the PCL-SiHA scaffolds with well-aligned structure (~82%). It is expected that novel hybrid scaffolds of PCL will improve tissue ingrowth in vivo due to hydrophilic SiHA microparticles in combination with randomly oriented and well-aligned PCL microfibers, which mimic the structure of extracellular matrix of bone tissue.

LanguageEnglish
Article number8907
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

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Silicates
Durapatite
Tissue Engineering
Tomography
Bone and Bones
Polymers
Synchrotrons
Trace Elements
Fourier Transform Infrared Spectroscopy
In Vitro Techniques
polycaprolactone
Mesenchymal Stromal Cells
Cell Adhesion
Extracellular Matrix
Regeneration
X-Rays

ASJC Scopus subject areas

  • General

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3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering : High-resolution tomography and in vitro study. / Shkarina, Svetlana; Shkarin, Roman; Weinhardt, Venera; Melnik, Elizaveta; Vacun, Gabriele; Kluger, Petra; Loza, Kateryna; Epple, Matthias; Ivlev, Sergei I.; Baumbach, Tilo; Surmeneva, Maria A.; Surmenev, Roman A.

In: Scientific Reports, Vol. 8, No. 1, 8907, 01.12.2018.

Research output: Contribution to journalArticle

Shkarina, Svetlana ; Shkarin, Roman ; Weinhardt, Venera ; Melnik, Elizaveta ; Vacun, Gabriele ; Kluger, Petra ; Loza, Kateryna ; Epple, Matthias ; Ivlev, Sergei I. ; Baumbach, Tilo ; Surmeneva, Maria A. ; Surmenev, Roman A. / 3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering : High-resolution tomography and in vitro study. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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