3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus

Alexander M. Demin, Alexandra G. Pershina, Vladimir V. Ivanov, Kseniya V. Nevskaya, Oleg B. Shevelev, Artyom S. Minin, Iliya V. Byzov, Alexey E. Sazonov, Victor P. Krasnov, Ludmila M. Ogorodova

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. Materials and methods: Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe3+ and Fe2+ salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis. Magnetic resonance properties of MNPs were investigated in vitro and in vivo. Results: The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g−1) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r2 was 271 mmol−1 s−1. To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus. Conclusion: The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage.

Original languageEnglish
Pages (from-to)4451-4463
Number of pages13
JournalInternational Journal of Nanomedicine
Volume11
DOIs
Publication statusPublished - 6 Sep 2016

Fingerprint

Opisthorchis
Magnetic resonance
Iron oxides
Liver
Nanoparticles
Magnetic Resonance Imaging
Imaging techniques
Transmission Electron Microscopy
ferric oxide
aminopropylsilane
Opisthorchiasis
Transmission electron microscopy
Fasciola hepatica
Flow cytometry
Annexin A5
Fourier Transform Infrared Spectroscopy
Hydrodynamics
Cytotoxicity
Coprecipitation
Contrast Media

Keywords

  • Alkoxysilane
  • Liver fluke
  • Magnetic nanoparticles
  • Magnetic resonance imaging

ASJC Scopus subject areas

  • Bioengineering
  • Biophysics
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus. / Demin, Alexander M.; Pershina, Alexandra G.; Ivanov, Vladimir V.; Nevskaya, Kseniya V.; Shevelev, Oleg B.; Minin, Artyom S.; Byzov, Iliya V.; Sazonov, Alexey E.; Krasnov, Victor P.; Ogorodova, Ludmila M.

In: International Journal of Nanomedicine, Vol. 11, 06.09.2016, p. 4451-4463.

Research output: Contribution to journalArticle

Demin, Alexander M. ; Pershina, Alexandra G. ; Ivanov, Vladimir V. ; Nevskaya, Kseniya V. ; Shevelev, Oleg B. ; Minin, Artyom S. ; Byzov, Iliya V. ; Sazonov, Alexey E. ; Krasnov, Victor P. ; Ogorodova, Ludmila M. / 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus. In: International Journal of Nanomedicine. 2016 ; Vol. 11. pp. 4451-4463.
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T1 - 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus

AU - Demin, Alexander M.

AU - Pershina, Alexandra G.

AU - Ivanov, Vladimir V.

AU - Nevskaya, Kseniya V.

AU - Shevelev, Oleg B.

AU - Minin, Artyom S.

AU - Byzov, Iliya V.

AU - Sazonov, Alexey E.

AU - Krasnov, Victor P.

AU - Ogorodova, Ludmila M.

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KW - Alkoxysilane

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